DIFFERENTIAL ACTIVITY OF INTERLEUKIN-1-ALPHA AND INTERLEUKIN-1-BETA IN THE STIMULATION OF THE IMMUNE-RESPONSE INVIVO
DIFFERENTIAL ACTIVITY OF INTERLEUKIN-1-ALPHA AND INTERLEUKIN-1-BETA IN THE STIMULATION OF THE IMMUNE-RESPONSE INVIVO
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DOI：
10.1002/eji.1830200213
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发表时间：
1990-02-01
影响因子：
5.4
通讯作者：
TAGLIABUE, A
中科院分区：
文献类型：
--
作者：
BORASCHI, D;VILLA, L;TAGLIABUE, A
人重组白细胞介素 (IL) 1.alpha 的生物活性。和IL 1.beta。在不同的生物系统中进行了比较。两种IL 1 形式在体外诱导鼠胸腺细胞和鼠T辅助克隆D10.G4.1增殖以及触发人皮肤成纤维细胞释放前列腺素E2方面具有同等活性。体内，IL 1.alpha。和IL 1.beta。兔和小鼠中的热原性相似，并且同样可以增加小鼠中急性期蛋白血清淀粉样蛋白 A 的循环水平。然而，只有IL 1.beta。显示出体内免疫刺激活性，因为它可以增加用 T 依赖性或 T 非依赖性抗原免疫的小鼠脾脏中产生特异性抗体的细胞的数量。尽管缺乏免疫刺激活性，IL 1.alpha。可以有效地竞争 IL1.β 诱导的免疫刺激，表明与 IL1 受体有效相互作用。因此，IL 1.beta。似乎在免疫反应的正向调节中具有重要作用，而 IL 1.alpha。可能充当 IL 1.beta 的下调剂。影响。
The biological activities of human recombinant interleukin (IL) 1.alpha. and IL 1.beta. were compared in different biological systems. The two IL 1 forms were equally active in vitro in inducing proliferation of murine thymocytes and of the murine T helper clone D10.G4.1, and in triggering release of prostaglandin E2 from human skin fibroblasts. In vivo, IL 1.alpha. and IL 1.beta. were similarly pyrogenic both in rabbits and mice, and could equally increase the circulating levels of the acute phase protein serum amyloid A in mice. However, only IL 1.beta. showed immunostimulatory activity in vivo, as it could enhance the number of specific antibody-producing cells in the spleen of mice immunized with either a T-dependent or a T-independent antigen. Although devoid of immunostimulatory activity, IL 1.alpha. could efficiently compete immunostimulation induced by IL1.beta., suggesting an effective interaction with the IL1 receptor. Thus, IL 1.beta. appears to have an important role in the positive regulation of immune responses, while IL 1.alpha. may act as down-regulator of the IL 1.beta. effect.