Molecular epidemiology of hepatitis B, C, D and E viruses among children in Moscow, Russia
Molecular epidemiology of hepatitis B, C, D and E viruses among children in Moscow, Russia
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DOI：
10.1016/j.jcv.2003.08.009
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发表时间：
2004-05-01
影响因子：
8.8
通讯作者：
Uchaikin, VF
中科院分区：
文献类型：
--
作者：
Abe, K;Hayakawa, E;Uchaikin, VF
背景：众所周知，HBV和HCV感染的流行率因地理区域而异。然而，在俄罗斯，到目前为止还没有足够的肝炎病毒分子流行病学信息。目的：为了调查俄罗斯各种肝炎病毒的特征，我们在莫斯科儿童中进行了肝炎病毒的分子流行病学调查，包括B型肝炎病毒（HBV）、丙型肝炎病毒（HCV）、丁型肝炎病毒（HDV）和戊型肝炎病毒（HEV），研究设计：374名受试者（年龄从1岁到14岁不等）的研究人群包括195名肝病患者和179名无肝病患者。巢式PCR法检测病毒DNA/RNA。用型特异性引物进行PCR检测HBV和HCV基因型。结果：急性肝炎、慢性肝炎、肝硬化患者中HBV、HCV的感染率分别为65.6%、15.9%。相比之下，在非肝病患者中，HBV和HCV的感染率分别为14.4%和0.6%。最常见的病毒基因型为HBV D型（85%）和HCV 1b型（79.3%）。在149例HBV DNA阳性儿童中，7例（4.7%）检出HDV RNA。此外，341名受试者中的HEV检测导致抗-HEVIgG的检测在62例（18.2%）。结论：我们的研究结果表明，H13病毒感染是广泛的莫斯科，并导致在该地区的儿童急性和慢性肝病的发病率很高。(C)2003 Elsevier B. V.保留所有权利。
Background: It is known that the prevalence of HBV and HCV infections vary according to geographical areas. However, in Russia, an adequate level of information on the molecular epidemiology of hepatitis viruses has not been available so far.Objectives: To investigate the characterization of various hepatitis viruses in Russia, we conducted molecular-based epidemiological survey of hepatitis viruses including hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus (HDV) and hepatitis E virus (HEV) among children in Moscow, Russia.Study design: The study population of 374 subjects (ranging in age from 1 to 14 years old) consisted of 195 patients with liver diseases and 179 patients without liver diseases. Viral DNA/RNA was determined by nested PCR. Genotyping of HBV and HCV were examined by PCR using type-specific primers. Anti-HEV antibody was assayed by ELISA.Results: The infection rate of each virus among patients with liver diseases including acute hepatitis, chronic hepatitis or cirrhosis was 65.6% for HBV and 15.9% for HCV. In contrast, among non-liver disease patients, the infection rates were 14.4% for HBV and 0.6% for HCV, respectively. The most common viral genotypes were type D (85%) of HBV and type 1b (79.3%) of HCV. HDV RNA was detected in 7 of 149 (4.7%) HBV DNA-positive children tested. Moreover, testing for HEV among 341 subjects resulted in the detection of anti-HEV IgG in 62 cases (18.2%).Conclusions: Our results suggest that Hl3V infection is widespread in Moscow and have led to a high incidence of acute and chronic liver diseases among children in this region. (C) 2003 Elsevier B.V. All rights reserved.