RhgKL and CcpA directly regulate the <i>rhiLFGN-rhgR-yesTUV</i> operon involved in the metabolism of rhamnogalacturonan type I in <i>Bacillus subtilis</i>
RhgKL and CcpA directly regulate the <i>rhiLFGN-rhgR-yesTUV</i> operon involved in the metabolism of rhamnogalacturonan type I in <i>Bacillus subtilis</i>
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RhgKL和CcpA直接调控<i>枯草芽孢杆菌</i>中参与鼠李半乳糖醛酸I型代谢的<i>rhiLFGN-rhgR-yesTUV</i>操纵子
DOI：
10.1093/bbb/zbac128
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hirooka Kazutake
中科院分区：
文献类型：
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作者：
R. Hu;A. Shimoyama;K. Fukase;Hirooka Kazutake
枯草芽孢杆菌rhiLFGN-rhgR-yesTUVWXYZ（原yesopqrstuvwxyz）基因簇包括代谢鼠李糖半乳糖酸I型（RG-I）的基因，这是一种主要的果胶成分，以及编码AraC/XylS转录激活因子的therhgRgene。与hilgene相邻的l - rhgkl（以前称为lmn）操纵子包括编码双组分调控系统的hgklgenes。报告分析显示，hil、yesW和yesl基因上游的3个启动子被RG-I诱导，并在培养基中被葡萄糖抑制。报道者的分析还表明，RhgL和RhgR参与了rg - i依赖性的hil启动子的诱导，CcpA介导hil启动子的分解代谢抑制。他们的体外实验表明，RhgL反应调节因子和CcpA复合物结合到hil启动子区域的每个位点。通过RT-PCR分析和对hilfgn - rhgr - yestuv启动子的不同性质，提示hilfgn - rhgr - yestuv基因是一个操纵子。
TheBacillus subtilis rhiLFGN-rhgR-yesTUVWXYZ(formerlyyesOPQRSTUVWXYZ) gene cluster includes genes for metabolizing rhamnogalacturonan type I (RG-I), a major pectin constituent, and therhgRgene encoding an AraC/XylS transcriptional activator. TheyesL-rhgKL(formerlyyesLMN) operon, adjacent to therhiLgene, includes therhgKLgenes encoding a two-component regulatory system. The reporter analyses showed that 3 promoters immediately upstream of therhiL, yesW, andyesLgenes were induced by RG-I and repressed by glucose in the medium. The reporter analyses also showed that RhgL and RhgR contribute to the RG-I-dependent induction of therhiLpromoter and that CcpA mediates the catabolite repression of therhiLandyesLpromoters. Thein vitroexperiments demonstrated that the RhgL response regulator and the CcpA complex bind to each site in therhiLpromoter region. The RT-PCR analysis and the different properties of therhiLandyesWpromoters suggested therhiLFGN-rhgR-yesTUVgenes as an operon.