Visualization of phosphorus re-translocation and phosphate transporter expression profiles in a shortened annual cycle system of poplar.
Visualization of phosphorus re-translocation and phosphate transporter expression profiles in a shortened annual cycle system of poplar.
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杨树缩短的年循环系统中磷重易位和磷酸盐转运蛋白表达谱的可视化。
DOI：
10.1111/pce.14319
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yuko Kurita;Satomi Kanno;Ryohei Sugita;Atsushi Hirose;Miwa Ohnishi;Ayumi Tezuka;Ayumi Deguchi;Kimitsune Ishizaki;Hidehiro Fukaki;Kei'ichi Baba;Atsushi J Nagano;Keitaro Tanoi;Tomoko M Nakanishi;Tetsuro Mimura
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文献类型：
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作者：
Shikata;H.;Yanagisawa,N.;Sato;Y.;Higashiyama;H.;Schwechheimer;C.;Yuko Kurita;Satomi Kanno;Ryohei Sugita;Atsushi Hirose;Miwa Ohnishi;Ayumi Tezuka;Ayumi Deguchi;Kimitsune Ishizaki;Hidehiro Fukaki;Kei'ichi Baba;Atsushi J Nagano;Keitaro Tanoi;Tomoko M Nakanishi;Tetsuro Mimura
磷（P）是植物生长必需的常量营养素。在落叶树中，磷从衰老的叶子中被重新利用，并在冬季储存在多年生组织中以供进一步生长。磷的年度内部循环和积累被认为是支持树木旺盛生长的重要策略。然而，磷的季节性重新移位的途径和这种运输的分子机制尚未阐明。在这里，我们展示了使用实时放射性同位素成像以及宏观和微观放射自显影技术可视化的季节性P重新易位路线。我们分析了在“缩短的年周期系统”下种植的杨树（Populus alba.L）中 P 的季节性重新易位，该系统模拟了实验室中的季节性物候。从生长期到衰老期，32P和/或33P的库组织从幼叶和顶端转移到下部茎和根。从叶子重新转移的放射性同位素P储存在韧皮部和木质部薄壁细胞中，并在休眠后重新分配给新芽。在同一系统中获得了磷酸盐转运蛋白（PHT1、PHT5 和 PHO1 家族）的季节性表达谱。我们的结果揭示了器官和组织水平上的季节性P重新易位途径，并为阐明其分子机制提供了立足点。
Phosphorus (P) is an essential macronutrient for plant growth. In deciduous trees, P is remobilized from senescing leaves and stored in perennial tissues during winter for further growth. Annual internal recycling and accumulation of P are considered an important strategy to support the vigorous growth of trees. However, the pathways of seasonal re‐translocation of P and the molecular mechanisms of this transport have not been clarified. Here we show the seasonal P re‐translocation route visualized using real‐time radioisotope imaging and the macro‐ and micro‐autoradiography. We analysed the seasonal re‐translocation P in poplar (Populus alba. L) cultivated under ‘a shortened annual cycle system’, which mimicked seasonal phenology in a laboratory. From growing to senescing season, sink tissues of32P and/or33P shifted from young leaves and the apex to the lower stem and roots. The radioisotope P re‐translocated from a leaf was stored in phloem and xylem parenchyma cells and redistributed to new shoots after dormancy. Seasonal expression profile of phosphate transporters (PHT1,PHT5andPHO1family) was obtained in the same system. Our results reveal the seasonal P re‐translocation routes at the organ and tissue levels and provide a foothold for elucidating its molecular mechanisms.