Early acquisition of Pneumocystis carinii in neonatal rats as evidenced by PCR and oral swabs
Early acquisition of Pneumocystis carinii in neonatal rats as evidenced by PCR and oral swabs
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DOI：
10.1128/ec.1.3.414-419.2002
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发表时间：
2002-06-01
期刊：
影响因子：
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通讯作者：
Cushion, MT
中科院分区：
文献类型：
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作者：
Icenhour, CR;Rebholz, SL;Cushion, MT
卡氏肺孢子虫的完整生命周期尚未确定，但越来越多的证据表明，哺乳动物宿主可能在生命早期获得这种生物体。在本研究中，通过扩增7组幼仔和母鼠的口腔拭子以及3只妊娠雌性大鼠剖腹产获得的胎儿组织中的卡氏肺孢子虫DNA，确定大鼠获得卡氏肺孢子虫的初始时间。从所有样品中提取的DNA通过使用针对卡氏肺吸虫线粒体大亚基rRNA的PCR引物扩增。80%（28/35）的幼仔在出生后2 h内产生扩增子; 97%（34/35）的幼仔在出生后24 h产生扩增子，所有连续采样的幼仔在出生后48 h产生扩增子。48例剖宫产胎儿或胎盘均未检出卡氏肺孢子虫扩增子。因此，卡氏肺孢子虫几乎在出生后立即获得，胎盘传播很少发生，如果有的话，在大鼠中。
The complete life cycle of Pneumocystis carinii has not been defined, but accumulating evidence suggests that the mammalian host may acquire this organism early in life. In the present study, the initial time of P. carinii acquisition was determined in rats by amplification of P. carinii DNA in oral swabs from seven sets of pups and dams and from fetal tissue obtained by cesarean section of three gravid female rats. DNA extracted from all samples was amplified by using PCR primers directed to the P. carinii mitochondrial large subunit rRNA. Amplicons were produced from 80% (28 of 35) of pups within 2 h after birth; from 97% (34 of 35) after 24 h, and in all of the serially sampled pups by 48 h. No P. carinii amplicons were produced from 48 fetuses or their placentae taken by cesarean section. Thus, P. carinii is acquired almost immediately after birth, and placental transmission occurs rarely, if ever, in rats.