The Use of an Artificial Skin Model to Study Transdermal Absorption of Drugs in Inflamed Skin
The Use of an Artificial Skin Model to Study Transdermal Absorption of Drugs in Inflamed Skin
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DOI：
10.1248/bpb.35.203
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发表时间：
2012-02-01
影响因子：
2
通讯作者：
Seki, Toshinobu
中科院分区：
文献类型：
--
作者：
Oshima, Shinji;Suzuki, Chihiro;Seki, Toshinobu
研究药物在炎症皮肤中的分布对安全有效地应用外用药物具有重要意义。在这里，氟比洛芬（FP）通过发炎的皮肤吸收进行了检查，在体内和皮肤模拟人工模型系统。模型皮肤系统由充当模型角质层的硅酮膜、充当存活皮肤模型的层压透析膜和2个微透析探针组成，一个用于测定FP浓度，一个充当模型血管。该模型系统可用于复杂渗透过程的定量评价。在体内实验中，FP吸收被抑制在大鼠与皮肤发炎诱导的2-角叉菜胶，酵母聚糖，酪蛋白的混合溶液的皮内注射。将牛血清白蛋白溶液置于模型皮肤系统中的透析膜之间，以模拟皮肤中的蛋白质浸出;结果表明，FP在发炎皮肤中的延迟吸收是由于与组织中浸出的血清蛋白结合。体内实验和模型皮肤系统的这种组合对于理解发炎和受损皮肤中的复杂现象是有用的，并且减少了实验动物的使用。
Studies on drug disposition in inflamed skin are important for safe and effective application of topical drugs. Here, the absorption of flurbiprofen (FP) through inflamed skin was examined in vivo and in a skin-mimicking artificial model system. The model skin system consisted of a silicone membrane acting as a model stratum corneum, laminated dialysis membranes acting as a model of viable skin, and 2 microdialysis probes one used for determination of FP concentration and one acting as a model vessel. This model system could be used for quantitative evaluation of complicated permeation processes. In the in vivo experiments, FP absorption was suppressed in rats with inflamed skin induced by an intracutaneous injection of a mixed solution of 2-carrageenan, zymosan, and casein. Bovine serum albumin solution was placed between the dialysis membranes in the model skin system to mimic protein leaching in skin; the results suggested that the delayed absorption of FP in inflamed skin was due to binding to serum proteins leaching in the tissue. Such a combination of in vivo experiments and a model skin system is useful for understanding complex phenomena in inflamed and damaged skin and reduces experimental animal use.