Unveiling equal importance of two 14-3-3 proteins for morphogenesis, conidiation, stress tolerance and virulence of an insect pathogen
Unveiling equal importance of two 14-3-3 proteins for morphogenesis, conidiation, stress tolerance and virulence of an insect pathogen
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揭示两种 14-3-3 蛋白对于昆虫病原体的形态发生、分生孢子形成、胁迫耐受性和毒力的同等重要性
DOI：
10.1111/1462-2920.12634
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发表时间：
2015-04-01
影响因子：
5.1
通讯作者：
Feng, Ming-Guang
中科院分区：
文献类型：
--
作者：
Liu, Qian;Li, Jin-Gen;Feng, Ming-Guang
两种保守的14-3-3蛋白质与苏氏酿酒酵母BMH1/2在丝状真菌中对酿酒酵母菌的蛋白质直接相关。在这里，我们表明BMH1和BMH2通过靶向许多蛋白质/酶，对Beauveria Bassiana的基本生物学和生理学贡献同样贡献。单个BMH缺失引起了另一个BMH的类似上调。 BMH2中BMH1和BMH2中BMH1的出色敲低（大约至91％）的表达式与单个缺失相比，BMH1中的BMH1表达式具有更严重的多种型缺陷，包括G（2）/M过渡，爆炸孢子大小，碳/氮利用，碳/氮利用，分生物，发芽，发芽，发芽，发芽，发芽，发芽对高渗透压，氧化，细胞壁应力，高温和UV-B的分生孢子公差辐照。所有缺失和缺失/敲低突变体在爆炸孢子产量和密度，菌丝分隔和细胞大小，对大多数化学胁迫和毒力的菌丝反应中均显示出相似的缺陷。随着与表型相关基因的转录本改变的变化，所有缺陷都是显而易见的，并且每个BMH互补都可以很好地恢复。我们在渗透和氧化应激下产生的BMH1-和BMH2特异性转录组揭示了在真菌基因组中至少两倍的差异表达的6％基因。其中许多在BMH1和BMH2中非常沮丧或抑郁。我们的发现提供了对两种14-3-3蛋白在丝状昆虫病原体中的功能和互补作用的详细见解。
Two conserved 14-3-3 proteins orthologous to Saccharomyces cerevisiaeBmh1/2 are poorly understood in filamentous fungi. Here we show that Bmh1 and Bmh2 contribute equally to the fundamental biology and physiology of Beauveria bassiana by targeting many sets of proteins/enzymes. Single Bmh deletion caused similar upregulation of another. Excellent knockdown (approximate to 91%) expressions of Bmh1 in Bmh2 and Bmh2 in Bmh1 resulted in equally more severe multiphenotypic defects than the single deletions, including G(2)/M transition, blastospore size, carbon/nitrogen utilization, conidiation, germination and conidial tolerances to high osmolarity, oxidation, cell wall stress, high temperature and UV-B irradiation. All the deletion and deletion/knockdown mutants showed similar defects in blastospore yield and density, hyphal septation and cell size, hyphal responses to most chemical stresses and virulence. All the defects were evident with altered transcripts of phenotype-related genes and well restored by each Bmh complementation. Our Bmh1- and Bmh2-specific transcriptomes generated under osmotic and oxidative stresses revealed up to 6% genes differentially expressed by at least twofold in the fungal genome. Many of those were greatly depressed or co-depressed in Bmh1 and Bmh2. Our findings provide a thorough insight into the functions and complementary effects of the two 14-3-3 proteins in the filamentous entomopathogen.