A coat protein on phagosomes involved in the intracellular survival of mycobacteria
A coat protein on phagosomes involved in the intracellular survival of mycobacteria
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DOI：
10.1016/s0092-8674(00)80754-0
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发表时间：
1999-05-14
期刊：
影响因子：
64.5
通讯作者：
Pieters, J
中科院分区：
文献类型：
--
作者：
Ferrari, G;Langen, H;Pieters, J
分枝杆菌是细胞内的病原体，可以在巨噬细胞吞噬体内存活，从而通过很大程度上未知的机制逃避宿主的防御策略。我们已经确定了WD重复宿主蛋白，该蛋白被活的而不是死的分枝杆菌招募并积极保留在吞噬体上。这种蛋白质称为TACO，代表吞噬体外壳的一种组分，其通常在吞噬体与溶酶体融合或成熟为溶酶体之前释放。在缺乏TACO的巨噬细胞中，分枝杆菌很容易被转运到溶酶体，然后被降解。非巨噬细胞中TACO的表达阻止了分枝杆菌的溶酶体递送并延长了其细胞内存活。因此，活分枝杆菌在吞噬体上主动保留TACO代表了阻止货物递送至溶酶体的机制，从而允许分枝杆菌在巨噬细胞内存活。
Mycobacteria are intracellular pathogens that can survive within macrophage phagosomes, thereby evading host defense strategies by largely unknown mechanisms. We have identified a WD repeat host protein that was recruited to and actively retained on phagosomes by living, but not dead, mycobacteria. This protein, termed TACO, represents a component of the phagosome coat that is normally released prior to phagosome fusion with or maturation into lysosomes. In macrophages lacking TACO, mycobacteria were readily transported to lysosomes followed by their degradation. Expression of TACO in nonmacrophages prevented lysosomal delivery of mycobacteria and prolonged their intracellular survival. Active retention of TACO on phagosomes by living mycobacteria thus represents a mechanism preventing cargo delivery to lysosomes, allowing mycobacteria to survive within macrophages.