Array painting using microdissected chromosomes to map chromosomal breakpoints
Array painting using microdissected chromosomes to map chromosomal breakpoints
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DOI：
10.1159/000098181
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Vermeesch, J. R.
中科院分区：
文献类型：
--
作者：
Backx, L.;Van Esch, H.;Vermeesch, J. R.
染色体重排断点的分子表征是鉴定候选疾病基因的成功策略。定位易位断点和重排染色体边界是劳动密集型和/或耗时的。在这里，我们提出了一种新的和快速的程序，通过杂交扩增的显微解剖衍生的异常染色体DNA到包含基因组克隆的阵列来绘制这种染色体断点。我们通过分子描绘五个小多余标记染色体（sSMC）的断点并绘制五个不同染色体易位的断点来说明该技术的潜力。版权所有(c) 2007 S. Karger AG，巴塞尔
Molecular characterization of breakpoints of chromosomal rearrangements is a successful strategy for the identification of candidate disease genes. Mapping translocation breakpoints and rearranged chromosomal boundaries is labor intensive and/or time consuming. Here, we present a novel and rapid procedure to map such chromosomal breakpoints by hybridizing amplified microdissection derived DNA of aberrant chromosomes to arrays containing genomic clones. We illustrate the potential of the technique by molecularly delineating the breakpoints in five small supernumerary marker chromosomes (sSMC) and mapping the breakpoints of five different chromosomal translocations. Copyright (c) 2007 S. Karger AG, Basel