Retinal attachment to the pigment epithelium. Linkage through an extracellular sheath surrounding cone photoreceptors.
Retinal attachment to the pigment epithelium. Linkage through an extracellular sheath surrounding cone photoreceptors.
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视网膜附着于色素上皮。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
A. M. Osterfeld
中科院分区：
文献类型：
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作者：
J. Hollyfield;Hugh H. Varner;M. Rayborn;A. M. Osterfeld
在正常人眼中，占据所谓视网膜下空间的感光细胞间基质在用铜蛋白蓝（一种选择性染色硫酸化聚阴离子的染料）处理后用电子显微镜检查。一个圆柱形的，细胞外成鞘域，由铜蛋白蓝阳性颗粒组成，观察周围的每个锥光感受器检查。棒周围不存在等同结构。周围的外周锥，单位颗粒，其中包括鞘，点状的形状，而在中央凹和小凹锥的颗粒更细长。基质域完全包围每个视锥光感受器，开始于外界膜，延伸超出视锥外节的尖端，并终止于色素上皮的顶面。近端的色素上皮，专门的扩展顶端微绒毛存在于管腔的基质鞘和较短的微绒毛似乎被嵌入鞘壁。当实验性地将视网膜从色素上皮分离并在固定和染色之前广泛洗涤时，视锥基质域仍然与视锥相关联，并且存在嵌入视锥基质鞘壁的远端尖端中的色素上皮细胞突起的破碎片段。这些观察结果表明，锥基质鞘是不溶性的，是密切相关的锥感光细胞和色素上皮的顶面。这些发现暗示视锥基质鞘是视网膜和色素上皮物理连接的桥梁。
The interphotoreceptor matrix, which occupies the so-called subretinal space in normal human eyes, was examined with electron microscopy after treatment with Cuprolinic Blue, a dye that selectively stains sulfated polyanions. A cylindrically shaped, extracellular ensheathing domain, composed of Cuprolinic Blue-positive particles, was observed surrounding each cone photoreceptor examined. Equivalent structures were not present around rods. Surrounding peripheral cones, the unit particles which comprised the sheath, were punctate in shape whereas in foveal and foveolar cones the particles were more elongate. A matrix domain completely enveloped each cone photoreceptor, beginning at the outer limiting membrane, extending beyond the tip of the cone outer segment, and ending at the apical surface of the pigment epithelium. Proximal to the pigment epithelium, specialized extensions of apical microvilli were present in the lumen of the matrix sheath and shorter microvilli appeared to be embedded in the sheath wall. When retinas were experimentally detached from the pigment epithelium and washed extensively before fixation and staining, the cone matrix domains remained associated with the cones, and broken fragments of pigment epithelial cell processes were present embedded in the distal tip of the cone matrix sheath wall. These observations indicate that the cone matrix sheath is insoluble and is closely associated with both the cone photoreceptor and the apical surface of the pigment epithelium. The implication of these findings is that the cone matrix sheath is a bridge through which the retina and pigment epithelium are physically attached.