Identification of kinin-related peptides in the disease vector, Rhodnius prolixus
Identification of kinin-related peptides in the disease vector, Rhodnius prolixus
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DOI：
10.1016/j.peptides.2010.10.002
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发表时间：
2011-03-01
期刊：
影响因子：
3
通讯作者：
Orchard, Ian
中科院分区：
文献类型：
--
作者：
Brugge, Victoria Te;Paluzzi, Jean-Paul;Orchard, Ian
我们已经使用了一种硅方法来识别来自嗜血载体的基因。Rhodnius prolixus，预计可产生昆虫激肽前原肽。前肽原长398个氨基酸，经过翻译后加工后可以潜在地产生大量的激肽相关肽。与其他昆虫激肽前体序列的比较表明，最大的保守性在C-末端区域的激肽肽。从激肽基因预测的多个肽在R.经MALDI-TOF MS MS分析，其主要成分为12种激肽和1种激肽前体肽（KPP）。这些肽中的六个以典型的昆虫激肽C-末端基序FX（1）X（2）WGamide为特征，并且这些肽中的五个也被发现为截短形式。鉴定了5种具有非典型但相似的FX（1）X（2）WA酰胺C-末端的肽。也存在具有C-末端DDNGamide基序的肽和许多非酰胺化肽。(C)2010年爱思唯尔公司All rights reserved.
We have used an in silica approach to identify a gene from the blood-gorging vector. Rhodnius prolixus, that is predicted to produce an insect kinin prepropeptide. The prepropeptide is 398 amino acids in length and can potentially produce a large number of kinin-related peptides following post-translational processing. A comparison with other insect kinin precursor sequences demonstrates greatest conservation at the C-terminal region of the kinin peptides. Multiple peptides predicted from the kinin gene are phenotypically expressed in R. prolixus, as revealed by MALDI-TOF MS MS, including 12 kinins and one kinin precursor peptide (KPP). Six of these peptides are characterized by the typical insect kinin C-terminal motif FX(1)X(2)WGamide and five of these are also found as truncated forms. Five peptides were identified with an atypical, though similar, FX(1)X(2)WAamide C-terminus. There is also peptide with a C-terminal DDNGamide motif and a number of non-amidated peptides. (C) 2010 Elsevier Inc. All rights reserved.