Laser Capture Microdissection
Laser Capture Microdissection
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DOI：
10.1007/978-1-61779-163-5
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Graeme I. Murray;S. Curran
中科院分区：
文献类型：
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作者：
Graeme I. Murray;S. Curran
激光显微切割技术彻底改变了研究人员，特别是病理学家对特定类型的正常和患病细胞进行分子分析的能力，并充分利用当前分子技术（包括 PCR、微阵列、下一代测序和蛋白质组学）的力量。本《分子生物学方法》第三版的主要目的是为读者提供实用建议，了解如何使用现有的不同激光显微切割系统并应用广泛的分子技术，在自己的实验室中成功进行基于组织的激光显微切割。各个章节包含对各个基于激光的显微切割系统的详细描述。书中描述的激光显微切割组织的下游应用包括下一代测序以及基因表达分析，包括微阵列和蛋白质组学的应用。编辑特别感谢所有贡献作者在各个章节中投入的大量时间和精力。系列编辑约翰·沃克 (John Walker) 在整个编辑过程中提供了专家指导，而施普林格的同事在处理所有与出版相关的问题时提供了很大的帮助。
Laser microdissection techniques have revolutionized the ability of researchers in general and pathologists in particular to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. The primary purpose of the third edition of this volume of Methods in Molecular Biology is to provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser-based microdissection systems. The downstream applications of the laser microdissected tissue described in the book include next generation sequencing as well as gene expression analysis including application to microarrays and proteomics. The editor is especially grateful to all the contributing authors for the considerable time and effort they have put into the individual chapters. The series editor, John Walker, has provided expert guidance through the editorial process while colleagues at Springer have been very helpful in dealing with all the publication-related issues.