Understanding the impact of crosslinked PCL/PEG/GeIMA electrospun nanofibers on bactericidal activity
Understanding the impact of crosslinked PCL/PEG/GeIMA electrospun nanofibers on bactericidal activity
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DOI：
10.1371/journal.pone.0209386
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发表时间：
2018-12-20
期刊：
影响因子：
3.7
通讯作者：
Lobo, Anderson Oliveira
中科院分区：
文献类型：
--
作者：
Machado De Paula, Mirian Michelle;Bassous, Nicole Joy;Lobo, Anderson Oliveira
在此，我们报告了基于三种不同聚合物聚己内酯（PCL）、聚乙二醇（PEG）和甲基丙烯酰明胶（GeIMA）的组合的静电纺丝纤维的设计，以及它们对三种不同细菌金黄色葡萄球菌（S.金黄色葡萄球菌（MRSA）、铜绿假单胞菌（P. aeruginosa）和耐甲氧西林金黄色葡萄球菌（MRSA）。我们评估了所生产的支架在紫外光交联前后的形态、化学结构和润湿性。结果表明，PEG和GeIMA掺入后，所开发的支架呈现亲水性。此外，它们能够显著减少革兰氏阳性、阴性和MRSA细菌，主要是在UV光交联（PCL：PEG：GelMa-UV）后。此外，我们进行了一系列的研究，通过蛋白质吸附研究和活性氧（ROS）水平的分析，以获得更好的机制理解的支架杀菌活性。此外，在大鼠体内皮下植入证实了我们设计的支架的生物相容性。
Herein, we report the design of electrospun ultrathin fibers based on the combination of three different polymers polycaprolactone (PCL), polyethylene glycol (PEG), and gelatin methacryloyl (GeIMA), and their potential bactericidal activity against three different bacteria Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), and Methicillin-resistant Staphylococcus aureus (MRSA). We evaluated the morphology, chemical structure and wettability before and after UV photocrosslinking of the produced scaffolds. Results showed that the developed scaffolds presented hydrophilic properties after PEG and GeIMA incorporation. Moreover, they were able to significantly reduce gram-positive, negative, and MRSA bacteria mainly after UV photocrosslinking (PCL:PEG:GelMa-UV). Furthermore, we performed a series of study for gaining a better mechanistic understanding of the scaffolds bactericidal activity through protein adsorption study and analysis of the reactive oxygen species (ROS) levels. Furthermore, the in vivo subcutaneous implantation performed in rats confirmed the biocompatibility of our designed scaffolds.