Extracellular pH sensing by plant cell-surface peptide-receptor complexes
Extracellular pH sensing by plant cell-surface peptide-receptor complexes
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DOI：
10.1016/j.cell.2022.07.012
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发表时间：
2022-09-01
期刊：
影响因子：
64.5
通讯作者：
Guo, Hongwei
中科院分区：
文献类型：
--
作者：
Liu, Li;Song, Wen;Guo, Hongwei
胞外pH是植物各种生物过程的重要调节因子。然而，植物如何感知细胞外pH值仍然不清楚。在这里，我们报告，植物细胞表面肽受体复合物可以作为胞外pH传感器。我们发现，模式触发免疫（PTI）显着碱化的酸性细胞外pH值的根尖分生组织（RAM）区域，这是必不可少的根分生组织生长因子1（RGF 1）介导的RAM的增长。细胞外碱化通过pH传感器磺基酪氨酸逐渐抑制RGF 1及其受体（RGFR）之间的酸依赖性相互作用。相反，细胞外碱化促进植物激发肽（Peps）通过pH传感器Glu/Asp与其受体（PEPR）的碱依赖性结合，从而促进免疫。RGFR和PEPR之间的结构域交换切换RAM生长的pH依赖性。因此，我们的研究结果揭示了植物肽受体复合物的细胞外pH传感机制，并提供了深入了解细胞外pH介导的RAM的生长和免疫调节。
The extracellular pH is a vital regulator of various biological processes in plants. However, how plants perceive extracellular pH remains obscure. Here, we report that plant cell-surface peptide-receptor com-plexes can function as extracellular pH sensors. We found that pattern-triggered immunity (PTI) dramatically alkalinizes the acidic extracellular pH in root apical meristem (RAM) region, which is essential for root meri-stem growth factor 1 (RGF1)-mediated RAM growth. The extracellular alkalinization progressively inhibits the acidic-dependent interaction between RGF1 and its receptors (RGFRs) through the pH sensor sulfotyrosine. Conversely, extracellular alkalinization promotes the alkaline-dependent binding of plant elicitor peptides (Peps) to its receptors (PEPRs) through the pH sensor Glu/Asp, thereby promoting immunity. A domain swap between RGFR and PEPR switches the pH dependency of RAM growth. Thus, our results reveal a mechanism of extracellular pH sensing by plant peptide-receptor complexes and provide insights into the extracellular pH-mediated regulation of growth and immunity in the RAM.