Co-downregulation of PTEN, KAI-1, and nm23-H1 tumor/metastasis suppressor proteins in non-small cell lung cancer.
Co-downregulation of PTEN, KAI-1, and nm23-H1 tumor/metastasis suppressor proteins in non-small cell lung cancer.
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DOI：
10.1016/j.anndiagpath.2003.11.002
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发表时间：
2004-02-01
影响因子：
2
通讯作者：
Ross, Jeffrey S
中科院分区：
文献类型：
--
作者：
Goncharuk, Viktor N;del-Rosario, Arthur;Ross, Jeffrey S
癌变的多步骤过程意味着多个分子缺陷的积累。肿瘤抑制基因和转移抑制基因的改变是肿瘤发生的重要环节。越来越多的实验证据表明，肿瘤转移/抑制基因及其产物的表达减少参与了多种人类恶性肿瘤的发生发展。在目前的研究中，我们将这一分析扩展到非小细胞肺癌(NSCLC)。检测抑癌基因PTEN、转移抑制基因nm23-H1和KAI-1在非小细胞肺癌中的表达及预后意义。应用抗PTEN、nm23H-1和KAI-1蛋白的单抗，对53例支气管腺癌和51例鳞癌的福尔马林固定石蜡包埋组织进行免疫组织化学染色。免疫组织化学结果与肿瘤分期、分级、淋巴结阳性、转移和患者生存有关。PTEN、nm23-H1和KAI-1在非小细胞肺癌中的表达差异有统计学意义(P
The multistep process of carcinogenesis implies the accumulation of multiple molecular defects. Alteration of tumor suppressor and metastasis suppressor genes are the important steps. Increasing experimental evidence indicates that decreased expression of tumor-metastasis/suppressor genes and gene products are involved in the progression of a variety of human malignancies. In the present study, we have extended this analysis to non-small cell lung carcinomas (NSCLC). The expression and prognostic significance of the tumor suppressor gene PTEN and metastasis suppressor genes nm23-H1 and KAI-1 was evaluated in NSCLCs. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissues from 53 bronchogenic adenocarcinomas and 51 squamous cell carcinomas using monoclonal antibodies against PTEN, nm23H-1, and KAI-1 proteins. Immunohistochemical results were correlated with tumor stage, grade, lymph nodes positivity, metastasis, and patient survival. Significant co-expression of PTEN, nm23-H1 and KAI-1 was observed in NSCLC (P