Miniaturized Devices for Bioluminescence Imaging in Freely Behaving Animals
Miniaturized Devices for Bioluminescence Imaging in Freely Behaving Animals
复制标题
用于自由行为动物生物发光成像的微型设备
DOI：
10.1109/embc44109.2020.9175375
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Moore, Christopher
中科院分区：
文献类型：
--
作者：
Celinskis, Dmitrijs;Friedman, Nina;Koksharov, Mikhail;Murphy, Jeremy;Gomez-Ramirez, Manuel;Borton, David;Shaner, Nathan;Hochgeschwender, Ute;Lipscombe, Diane;Moore, Christopher
In vivo fluorescence miniature microscopy has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, fluorescence imaging suffers from autofluorescence, phototoxicity, photobleaching and non- homogeneous illumination artifacts. These factors limit the quality and time course of data collection. Bioluminescence provides an alternative kind of activity-dependent light indicator. Bioluminescent calcium indicators do not require light input, instead generating photons through chemiluminescence. As such, limitations inherent to the requirement for light presentation are eliminated. Further, bioluminescent indicators also do not require excitation light optics: the removal of these components should make a lighter and lower cost microscope with fewer assembly parts. While there has been significant recent progress in making brighter and faster bioluminescence indicators, the advances in imaging hardware have not yet been realized. A hardware challenge is that despite potentially higher signal-to-noise of bioluminescence, the signal strength is lower than that of fluorescence. An open question we address in this report is whether fluorescent miniature microscopes can be rendered sensitive enough to detect bioluminescence. We demonstrate this possibility in vitro and in vivo by implementing optimizations of the UCLA fluorescent miniscope v3.2. These optimizations yielded a miniscope (BLmini) which is 22% lighter in weight, has 45% fewer components, is up to 58% less expensive, offers up to 15 times stronger signal and is sensitive enough to capture spatiotemporal dynamics of bioluminescence in the brain with a signal-to-noise ratio of 34 dB.
影响因子：
2.9
作者：
K. Kogure;O. Alonso
通讯作者：
O. Alonso
DOI：
10.1101/2020.04.21.044982
发表时间：
2020-04
期刊：
bioRxiv
影响因子：
--
作者：
Ankit Raghuram;Fan Ye;Jesse K. Adams;Nathan Christopher Shaner;Jacob T. Robinson;A. Veeraraghavan
通讯作者：
Ankit Raghuram;Fan Ye;Jesse K. Adams;Nathan Christopher Shaner;Jacob T. Robinson;A. Veeraraghavan