Effects of atrazine exposure on male reproductive performance in Drosophila melanogaster.
Effects of atrazine exposure on male reproductive performance in Drosophila melanogaster.
复制标题
阿特拉津暴露对果蝇雄性生殖性能的影响。
DOI：
10.1016/j.jinsphys.2014.11.002
复制
发表时间：
2015
影响因子：
2.2
通讯作者：
Fiumera,AnthonyC
中科院分区：
文献类型：
--
作者：
Vogel,Andrea;Jocque,Harper;Sirot,LauraK;Fiumera,AnthonyC
阿特拉津是一种常用的除草剂，用于控制农业环境中的阔叶杂草。然而，它可以对各种脊椎动物物种的雄性生殖性能产生负面影响。然而，人们对阿特拉津对无脊椎动物的影响知之甚少。在这项研究中，我们调查了几种不同浓度的幼虫阿特拉津暴露在成年雄性果蝇的生殖性能的措施的影响。阿特拉津暴露对雄性的交配能力和他的伴侣在成功交配时产卵的数量有显着影响。在竞争条件下，暴露的雄性在第一次与双重交配的雌性交配时，也会产生较小比例的后代。阿特拉津暴露没有可测量的影响男性的能力，以防止交配的女性交配到另一个男性或后代的比例，当暴露的男性是第二个男性交配。暴露上调表达的一个男性生殖基因，ovulin，但对另一个，性肽的表达没有影响。暴露的男性生产和转移更多的性肽蛋白的女性在交配过程中，但排卵蛋白水平没有受到影响。一般来说，我们观察到非单调的反应，中等暴露水平显示出最大的男性生殖性能下降。这项研究表明，阿特拉津暴露会影响雄性昆虫的生殖性能，未来的研究应旨在了解暴露的健身效果的分子机制。
Atrazine is a commonly utilized herbicide to control broadleaf weeds in the agricultural setting. It can, however, have negative effects on male reproductive performance in a variety of vertebrate species. Much less is known, however, about the effects of atrazine on invertebrates. In this study, we investigated the effects of several different concentrations of larval atrazine exposure on measures of reproductive performance in adult maleDrosophila melanogaster. Atrazine exposure had significant effects on a male’s mating ability and the number of eggs his partner laid when he was successful at mating. Exposed males also sired a smaller proportion of the offspring under competitive conditions when they were the first male to mate to a doubly mated female. Atrazine exposure had no measurable effect on a male’s ability to prevent a mated female from mating to another male or on the proportion of offspring sired when the exposed males were the second male to mate. Exposure upregulated expression of one male reproductive gene,ovulin, but had no effect on expression of another,sex peptide. Exposed males produced and transferred more sex peptide protein to the female during mating but ovulin protein levels were not affected. In general, we observed non-monotonic responses such that the intermediate exposure levels showed the largest reduction in male reproductive performance. This study suggests that atrazine exposure affects male reproductive performance in insects and future studies should aim to understand the molecular mechanisms underlying the fitness effects of exposure.