Draft genome of the big-headed turtle Platysternon megacephalum
Draft genome of the big-headed turtle Platysternon megacephalum
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DOI：
10.1038/s41597-019-0067-9
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发表时间：
2019-05-16
期刊：
影响因子：
9.8
通讯作者：
Gong, Shiping
中科院分区：
文献类型：
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作者：
Cao, Dainan;Wang, Meng;Gong, Shiping
大头龟Platysternon egacephalum是单种科Platysternidae中唯一的成员，具有许多明显的特征，包括特大头、长尾、扁平的甲壳，以及对低水温环境的偏好。我们基于Illumina HiSeq X基因组测序平台对一只成年雄性大头龟进行了全基因组测序、组装和基因注释。我们产生了大约497.1 GB的原始测序数据(x208.9深度)，并产生了一个总长度为2.32 GB的基因组草稿，重叠群和支架N50的大小分别为41.8kb和7.22Mb。我们还鉴定了924Mb(39.84%)的重复序列、25995个蛋白质编码基因和19,177个非编码RNA。我们获得了第一个大头龟的从头基因组，这些数据将对进一步了解和探索导致其独特形态和生理特征的基因组创新和分子机制至关重要。
The big-headed turtle, Platysternon megacephalum, as the sole member of the monotypic family Platysternidae, has a number of distinct characteristics including an extra-large head, long tail, flat carapace, and a preference for low water temperature environments. We performed whole genome sequencing, assembly, and gene annotation of an adult male big-headed turtle based on the Illumina HiSeq X genomic sequencing platform. We generated similar to 497.1 Gb of raw sequencing data (x208.9 depth) and produced a draft genome with a total length of 2.32 Gb and contig and scaffold N50 sizes of 41.8 kb and 7.22 Mb, respectively. We also identified 924 Mb (39.84%) of repetitive sequences, 25,995 protein-coding genes, and 19,177 non-coding RNAs. We generated the first de novo genome of the big-headed turtle; these data will be essential to the further understanding and exploration of the genomic innovations and molecular mechanisms contributing to its unique morphology and physiological features.