Plant Cell Vacuoles: Staining and Fluorescent Probes
Plant Cell Vacuoles: Staining and Fluorescent Probes
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DOI：
10.1007/978-1-4939-7856-4_5
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Brandizzi, Federica
中科院分区：
文献类型：
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作者：
Stefano, Giovanni;Renna, Luciana;Brandizzi, Federica
在植物细胞中，液泡对于生长和发育极其重要，并影响光合作用、呼吸和蒸腾等重要的细胞功能。植物细胞含有溶解液泡和储存液泡，其大小可能因细胞类型和组织发育阶段而异。液泡的主要作用之一是调节细胞膨胀以响应不同的刺激。因此，研究液泡的形态、动力学和生理学对于提高整个植物细胞生物学的知识至关重要。荧光探针的可用性允许以多种方式标记液泡。当使用市售化学染料时，这些可能很快，或者在基于指向液泡膜（液泡膜）或液泡腔的蛋白质的特定遗传编码标记的情况下相对较慢。这些方法中的任何一种都提供了有关液泡形态和生理学的有用信息。
In plant cells, vacuoles are extremely important for growth and development, and influence important cellular functions as photosynthesis, respiration, and transpiration. Plant cells contain lytic and storage vacuoles, whose size can be different depending on cell type and tissue developmental stage. One of the main roles of vacuoles is to regulate the cell turgor in response to different stimuli. Thus, studying the morphology, dynamics, and physiology of vacuole is fundamentally important to advance knowledge in plant cell biology at large. The availability of fluorescent probes allows marking vacuoles in multiple ways. These may be fast, when using commercially available chemical dyes, or relatively slow, in the case of specific genetically encoded markers based on proteins directed either to the membrane of the vacuole (tonoplast) or to the vacuole lumen. Any of these approaches provides useful information about the morphology and physiology of the vacuole.