Amount of Bone Lengthening Affects Blood Flow Recovery and Bone Mineralization After Distraction Osteogenesis in a Canine Cleft Palate Model
Amount of Bone Lengthening Affects Blood Flow Recovery and Bone Mineralization After Distraction Osteogenesis in a Canine Cleft Palate Model
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DOI：
10.1597/08-019.1
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发表时间：
2010-05-01
期刊：
影响因子：
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通讯作者：
Moriyama, Keiji
中科院分区：
文献类型：
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作者：
Aoki, Asato;Kawamoto, Tatsuo;Moriyama, Keiji
目的：牵张成骨术已应用于颅面区。为了减少唇腭裂患者的裂隙宽度，将牙槽骨向裂隙方向分散。然而，没有报道描述该区域牵张成骨所能达到的延长量的限制。因此，我们使用犬腭裂模型研究不同程度牵张成骨后的愈合过程。方法：在上腭处造10mm骨缺损。准备包括犬在内的骨段，以1 mm/d的速度移位到缺损区域6或10天，分为两组（6和10 mm组）。用多普勒血流仪监测犬髓血流量100 d。然后处死动物，对再生骨面积进行影像学和组织学评价。经Mann-Whitney秩次检验证实有统计学意义。结果：6 mm组髓血流量恢复较10 mm组早。外围定量计算机断层扫描测量再生骨的皮质骨密度，6毫米组明显大于10毫米组。6mm组组织学切片上再生骨的数量也显著增加。结论：我们清楚地表明，愈合进展取决于牵张成骨的程度，强调了在肺泡区有限的牵张成骨的重要性。
Objectives: Distraction osteogenesis has been applied to the craniofacial region. To reduce the cleft width of patients with cleft lip and palate, alveolar bones are distracted toward the cleft. However, no reports have described limitations to the amount of lengthening that can be achieved by distraction osteogenesis in this area. Therefore, we investigated the healing process following different extents of distraction osteogenesis using a canine cleft palate model.Methods: A 10-mm bone defect was made in the palates. A bony segment including the canine was prepared and translocated into the defect area at a rate of 1 mm/d for 6 or 10 days, resulting in two groups (6- and 10-mm groups). Canine pulpal blood flow was monitored for 100 days with Doppler flowmetry. Then, the animals were sacrificed and the regenerated bone area was evaluated radiologically and histologically. Statistical significance was confirmed with the Mann-Whitney rank test.Results: Pulpal blood flow in the 6-mm group recovered to original levels earlier than in the 10-mm group. Cortical bone density in the regenerated bone, measured by peripheral quantitative computed tomography, was significantly greater in the 6-mm group than in the 10-mm group. The amount of regenerated bone in histologic sections was also significantly greater in the 6-mm group.Conclusion: We clearly showed that healing progress depends on the extent of distraction osteogenesis, highlighting the importance of limited distraction osteogenesis in the alveolar area.