Cell directional migration and oriented division on three-dimensional laser-induced periodic surface structures on polystyrene.
Cell directional migration and oriented division on three-dimensional laser-induced periodic surface structures on polystyrene.
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DOI：
10.1016/j.biomaterials.2007.12.047
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发表时间：
2008-05
期刊：
影响因子：
14
通讯作者：
Xuefeng Wang;C. Ohlin;Q. Lu;Jun Hu
中科院分区：
文献类型：
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作者：
Xuefeng Wang;C. Ohlin;Q. Lu;Jun Hu
动物组织中的细胞外基质除了执行各种其他重要功能外，通常还为细胞提供三维结构支持。利用波长为266nm的偏振激光在光滑的聚苯乙烯薄膜上制备了波状亚微米周期性表面结构。大鼠C6胶质瘤细胞在激光照射的聚苯乙烯上具有平行于LIPSS方向的定向迁移和定向分裂。而大鼠C6胶质瘤细胞在光滑聚苯乙烯上的迁移呈三步侵袭循环，迁移速度快于激光照射聚苯乙烯。此外，通过免疫染色粘着斑激酶在人上皮癌HeLa细胞中检查的粘着斑在光滑的聚苯乙烯上是点状的，而在激光照射的聚苯乙烯上是破折号状的。我们假设，激光照射的聚苯乙烯上的LIPSS作为各向异性和持久的机械刺激，引导细胞各向异性扩散，迁移和分裂通过局灶性粘连。
The extracellular matrix in animal tissues usually provides a three-dimensional structural support to cells in addition to performing various other important functions. In the present study, wavy submicrometer laser-irradiated periodic surface structures (LIPSS) were produced on a smooth polystyrene film by polarized laser irradiation with a wavelength of 266nm. Rat C6 glioma cells exhibited directional migration and oriented division on laser-irradiated polystyrene, which was parallel to the direction of LIPSS. However, rat C6 glioma cells on smooth polystyrene moved in a three-step invasion cycle, with faster migration speed than that on laser-irradiated polystyrene. In addition, focal adhesions examined by immunostaining focal adhesion kinase in human epithelial carcinoma HeLa cells were punctuated on smooth polystyrene, whereas dash-like on laser-irradiated polystyrene. We hypothesized that LIPSS on laser-irradiated polystyrene acted as an anisotropic and persistent mechanical stimulus to guide cell anisotropic spreading, migration and division through focal adhesions.