Multisample analysis using an array of microreactors for an alternating-current field-enhanced latex immunoassay.
Multisample analysis using an array of microreactors for an alternating-current field-enhanced latex immunoassay.
复制标题
使用一系列微反应器进行交流场增强乳胶免疫分析的多样品分析。
DOI：
10.1021/ac00078a003
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发表时间：
1994
影响因子：
7.4
通讯作者：
I. Karube
中科院分区：
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作者：
M. Song;K. Iwata;M. Yamada;K. Yokoyama;T. Takeuchi;E. Tamiya;I. Karube
为了开发一种用于亚微升样品乳胶免疫分析的快速多样品分析系统，采用光刻、各向异性蚀刻和薄金膜沉积等微加工技术制作了一系列微反应器。单孔免疫反应室容积为0.4微升。采用交流电场提高胶乳的凝集反应速率。通过施加交流电场1min，可以同时测定多个样品中的甲胎蛋白。该系统的检出限约为10 pg/mL。
To develop a rapid and multisample analysis system for latex immunoassay with submicroliters of sample, an array of microreactors were fabricated using micromachining techniques including photolithography, anisotropic etching, and thin gold film deposition. The chamber volume for immunoreactions of a single well was 0.4 microL. An alternating-current (ac) field was used to enhance the rate of the latex agglutination reaction. By applying an ac field for 1 min, alpha-fetoprotein in several samples could be determined simultaneously. The detection limit in this system was approximately 10 pg/mL.
Eiichi TAMIYA：“使用抗体结合乳胶珠对人免疫球蛋白 G 进行脉冲免疫测定”生物传感器。
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