Preparation and characterization of a stratum corneurn substitute for in vitro percutaneous penetration studies
Preparation and characterization of a stratum corneurn substitute for in vitro percutaneous penetration studies
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DOI：
10.1016/j.bbamem.2006.04.001
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发表时间：
2006-05-01
影响因子：
3.4
通讯作者：
Bouwstra, Joke
中科院分区：
文献类型：
--
作者：
de Jager, Miranda;Groenink, Wouter;Bouwstra, Joke
细胞间角质层（SC）脂质形成物质通过皮肤扩散的主要屏障。覆盖有合成SC脂质的多孔基质将是研究经皮渗透的有吸引力的模型，从而取代天然的人SC。前提是这种角质层替代品（SCS）制备有均匀的脂质组成和层厚度。此外，脂质的组织和方向应该类似于SC。本研究的目的是研究喷枪喷涂装置的效用，以制备由胆固醇，神经酰胺和游离脂肪酸的聚碳酸酯过滤器上的SCS。结果表明，溶剂混合物和脂质浓度的适当选择对于实现所施加的脂质在过滤器表面上的均匀分布至关重要。只有当适当选择平衡条件时，才能获得光滑且紧密堆积的脂质层。SCS具有两个结晶层状相的周期性类似于那些存在于本机SC。这些层状的取向主要是平行于聚碳酸酯过滤器的表面，这类似于细胞间SC脂质的取向。总之，喷枪技术能够生成均匀的SCS，其最终可用作预测性体外经皮渗透模型。(c)2006 Elsevier B.V.保留所有权利。
The intercellular stratum comeum (SC) lipids form the main barrier for diffusion of substances through the skin. A porous substrate covered with synthetic SC lipids would be an attractive model to study percutaneous penetration, hereby replacing native human SC. Prerequisite is that this stratum comeum substitute (SCS) is prepared with a uniform lipid composition and layer thickness. Furthermore, the lipid organization and orientation should resemble that in SC. The objective of this study was to investigate the utility of an airbrush spraying device to prepare a SCS composed of cholesterol, ceramides and free fatty acids on a polycarbonate filter. The results demonstrate that a proper choice of solvent mixture and lipid concentration is crucial to achieve a uniform distribution of the applied lipids over the filter surface. A smooth and tightly packed lipid layer is only obtained when the equilibration conditions are appropriately chosen. The SCS possesses two crystalline lamellar phases with periodicities similar to those present in native SC. The orientation of these lamellae is mainly parallel to the surface of the polycarbonate filter, which resembles the orientation of the intercellular SC lipids. In conclusion, the airbrush technique enables generation of a homogeneous SCS, which ultimately may function as a predictive in vitro percutaneous penetration model. (c) 2006 Elsevier B.V. All rights reserved.