Characterization of a porcine intestinal epithelial cell line for influenza virus production.
Characterization of a porcine intestinal epithelial cell line for influenza virus production.
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DOI：
10.1099/vir.0.044388-0
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发表时间：
2012-09
期刊：
影响因子：
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通讯作者：
Zhi Sun;Victor C. Huber;K. McCormick;R. Kaushik;A. Boon;Longchao Zhu;B. Hause;R. Webby;Ying Fang
中科院分区：
文献类型：
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作者：
Zhi Sun;Victor C. Huber;K. McCormick;R. Kaushik;A. Boon;Longchao Zhu;B. Hause;R. Webby;Ying Fang
我们已经开发了一种猪小肠上皮细胞系，命名为SD-PJEC，用于流感病毒的繁殖。SD-PJEC细胞系是最初来源于新生仔猪空肠的IPEC-J2细胞系的亚克隆。我们的研究结果表明，SD-PJEC是一种上皮来源的细胞系，优先表达Sia 2 - 6 Gal修饰的寡糖受体。该细胞系允许人和猪流感A病毒和一些禽流感病毒感染，但对人源流感B病毒的生长支持较差。猪源流感病毒在这些细胞中的繁殖导致感染后最初24小时内快速生长，滴度范围为4至8 log（10）TCID（50）ml（-1）。进一步测试了SD-PJEC细胞系作为Madin-Darby犬肾（MDCK）细胞与293 T细胞联合使用的潜在替代细胞系，用于使用反向遗传学系统拯救猪源流感病毒。重组病毒A/swine/北卡罗来纳州/18161/02（H1 N1）和A/swine/Texas/4199-2/98（H3 N2）的病毒滴度分别为7和8.25 log（10）TCID（50）ml（-1）。这种猪特异性细胞系的可用性代表了猪源流感病毒研究和生长的更相关底物。
We have developed a porcine intestine epithelial cell line, designated SD-PJEC for the propagation of influenza viruses. The SD-PJEC cell line is a subclone of the IPEC-J2 cell line, which was originally derived from newborn piglet jejunum. Our results demonstrate that SD-PJEC is a cell line of epithelial origin that preferentially expresses receptors of oligosaccharides with Sia2-6Gal modification. This cell line is permissive to infection with human and swine influenza A viruses and some avian influenza viruses, but poorly support the growth of human-origin influenza B viruses. Propagation of swine-origin influenza viruses in these cells results in a rapid growth rate within the first 24 h post-infection and the titres ranged from 4 to 8 log(10) TCID(50) ml(-1). The SD-PJEC cell line was further tested as a potential alternative cell line to Madin-Darby canine kidney (MDCK) cells in conjunction with 293T cells for rescue of swine-origin influenza viruses using the reverse genetics system. The recombinant viruses A/swine/North Carolina/18161/02 (H1N1) and A/swine/Texas/4199-2/98 (H3N2) were rescued with virus titres of 7 and 8.25 log(10) TCID(50) ml(-1), respectively. The availability of this swine-specific cell line represents a more relevant substrate for studies and growth of swine-origin influenza viruses.