Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G
Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G
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DOI：
10.1371/journal.pone.0177433
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发表时间：
2017-05-24
期刊：
影响因子：
3.7
通讯作者：
Borca, Manuel V.
中科院分区：
文献类型：
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作者：
Holinka, Lauren G.;O'Donnell, Vivian;Borca, Manuel V.
在猪瘟流行地区和无疫区暴发期间，使用猪瘟减毒活疫苗进行预防性接种是控制该病的一种非常有效的方法。这些疫苗在接种后的早期赋予针对疾病的有效保护，尽管介导保护的机制的特征很差。在这里，我们介绍了接种我们内部开发的减毒活标记疫苗FlagT 4Gv后发生的事件。我们先前报道，FlagT 4Gv肌肉内（IM）给药早在疫苗接种后7天就赋予针对强毒CSFV（BICv）的鼻内攻击的有效保护。在这里，我们报告FlagT 4Gv能够早在接种后三天诱导针对疾病的保护。FlagT 4Gv接种动物组织的免疫组织化学检测显示，接种后第3天，扁桃体被疫苗病毒定殖。FlagT 4Gv感染后3天用BICv鼻内（IN）激发的FlagT 4Gv接种动物的扁桃体中完全不存在BICv，证实FlagT 4Gv接种可赋予无菌免疫力。对接种疫苗的动物中19种不同细胞因子的全身水平的分析表明，与模拟感染的对照相比，FlagT 4Gv接种后三天IFN-α增加。
Prophylactic vaccination using live attenuated classical swine fever (CSF) vaccines has been a very effective method to control the disease in endemic regions and during outbreaks in previously disease-free areas. These vaccines confer effective protection against the disease at early times post-vaccination although the mechanisms mediating the protection are poorly characterized. Here we present the events occurring after the administration of our in-house developed live attenuated marker vaccine, FlagT4Gv. We previously reported that FlagT4Gv intramuscular (IM) administered conferred effective protection against intranasal challenge with virulent CSFV (BICv) as early as 7 days post-vaccination. Here we report that FlagT4Gv is able to induce protection against disease as early as three days post-vaccination. Immunohistochemical testing of tissues from FlagT4Gv-inoculated animals showed that tonsils were colonized by the vaccine virus by day 3 post-inoculation. There was a complete absence of BICv in tonsils of FlagT4Gv-inoculated animals which had been intranasal (IN) challenged with BICv 3 days after FlagT4Gv infection, confirming that FlagT4Gv inoculation confers sterile immunity. Analysis of systemic levels of 19 different cytokines in vaccinated animals demonstrated an increase of IFN-alpha three days after FlagT4Gv inoculation compared with mock infected controls.