Fine mapping of Co, a gene controlling columnar growth habit located on apple (Malus x domestica Borkh.) linkage group 10
Fine mapping of Co, a gene controlling columnar growth habit located on apple (Malus x domestica Borkh.) linkage group 10
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DOI：
10.1111/j.1439-0523.2012.01985.x
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发表时间：
2012-10-01
期刊：
影响因子：
2
通讯作者：
Abe, Kazuyuki
中科院分区：
文献类型：
--
作者：
Moriya, Shigeki;Okada, Kazuma;Abe, Kazuyuki
摘要摘要树结构和枝条形态是苹果生产系统中的重要因素，有2张图3张表。由于柱状生长习惯有利于高密度种植和节省劳动力的水果生产方法，柱状苹果育种计划正在全球范围内推广。柱状生长习性由连锁群10上的1对显性基因Co控制。对31个群体的1000株F1后代进行了Co的精细定位。根据苹果金冠苹果基因组序列开发了新的SSR标记，并对其与Co连锁进行了分析。对Co附近发生重组事件的22个后代进行了图解基因分型，结果表明，与Co共分离的3个SSR标记(Mdo.chr10.12、Mdo.chr10.13和Mdo.chr10.14)非常适合于标记辅助选择，因为与Co连锁的等位基因大小与现有苹果育种种质的共同祖先中发现的不同。Co区被限定为苹果基因组中一个196 kb的区域，介于标记Mdo.chr10.11和Mdo.chr10.15之间。
With 2 figures and 3 tables Abstract Tree architecture and shoot morphology are important factors in apple production systems. Because a columnar growth habit facilitates high-density planting and labour-saving fruit production methods, columnar apple breeding programmes are spreading worldwide. Columnar growth habit is controlled by a single dominant gene, Co, on linkage group 10. Fine mapping of Co was conducted using 1000 F1 progeny plants from 31 populations. Novel simple sequence-repeat (SSR) markers were developed based on the genome sequence of apple Golden Delicious and analysed for linkage to Co. Graphical genotyping of 22 progeny with recombination event in the vicinity of Co identified three SSR markers (Mdo.chr10.12, Mdo.chr10.13 and Mdo.chr10.14) that co-segregated with Co. Mdo.chr10.12 and Mdo.chr10.14 are well suited for marker-assisted selection because the sizes of the alleles linked to Co are different from those found in common ancestors of current apple breeding germplasm. The Co region was delimited to a 196-kb region of the apple genome, between markers Mdo.chr10.11 and Mdo.chr10.15.