Consequence of a sudden wind event on the dynamics of a coastal phytoplankton community: an insight into specific population growth rates using a single cell high frequency approach.
Consequence of a sudden wind event on the dynamics of a coastal phytoplankton community: an insight into specific population growth rates using a single cell high frequency approach.
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DOI：
10.3389/fmicb.2014.00485
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发表时间：
2014
影响因子：
5.2
通讯作者：
Grégori GJ
中科院分区：
文献类型：
--
作者：
Dugenne M;Thyssen M;Nerini D;Mante C;Poggiale JC;Garcia N;Garcia F;Grégori GJ
浮游植物是海洋生态系统的重要组成部分。它负责大多数海洋初级生产，特别是在富营养化泻湖，在那里它经常开花。由于它们对环境非常敏感，因此必须在不同的时间尺度上观察这些微生物群落的动态，然而，评估短期变异性通常是传统监测方法无法达到的。为了克服这些限制，我们在地中海最大的泻湖之一Berre泻湖（法国东南部）建立了一个Cytosense自动流式细胞仪（Cytobuoy b.v.），用于高频监测浮游植物的组成、丰度、细胞大小和色素含量。2011年10月，它记录了12组微型、纳米和微型浮游植物的细胞光学特性。在细胞周期中，团簇光学特性的日常变化与显微镜成像观察到的个体变化一致。因此，我们采用了Sosik等人开发的尺寸结构矩阵种群模型的适应性来处理集群的单细胞分析，并估计了2个鞭毛藻种群在强风事件之前、期间和之后的分裂率。估计每日原位簇生长速率的增加表明，细胞的生理变化可以胜过丰度的反应。
Phytoplankton is a key component in marine ecosystems. It is responsible for most of the marine primary production, particularly in eutrophic lagoons, where it frequently blooms. Because they are very sensitive to their environment, the dynamics of these microbial communities has to be observed over different time scales, however, assessment of short term variability is often out of reach of traditional monitoring methods. To overcome these limitations, we set up a Cytosense automated flow cytometer (Cytobuoy b.v.), designed for high frequency monitoring of phytoplankton composition, abundance, cell size, and pigment content, in one of the largest Mediterranean lagoons, the Berre lagoon (South-Eastern France). During October 2011, it recorded the cell optical properties of 12 groups of pico-, nano-, and microphytoplankton. Daily variations in the cluster optical properties were consistent with individual changes observed using microscopic imaging, during the cell cycle. We therefore used an adaptation of the size-structured matrix population model, developed by Sosik et al. to process the single cell analysis of the clusters and estimate the division rates of 2 dinoflagellate populations before, during, and after a strong wind event. The increase in the estimated in situ daily cluster growth rates suggest that physiological changes in the cells can prevail over the response of abundance.
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影响因子：
2.5
作者：
Gisselson, LÅ;Granéli, E;Carlsson, P
通讯作者：
Carlsson, P
影响因子：
2.4
作者：
KIM, KT
通讯作者：
KIM, KT
影响因子：
1.4
作者：
Figueroa, Rosa Isabel;Bravo, Isabel;Morono, Angeles
通讯作者：
Morono, Angeles
影响因子：
1.9
作者：
ACKLESON, SG;SPINRAD, RW
通讯作者：
SPINRAD, RW
影响因子：
56.9
作者：
Field, CB;Behrenfeld, MJ;Falkowski, P
通讯作者：
Falkowski, P