A serine/threonine kinase gene BcATG1 is involved in conidiation and sclerotial development in Botrytis cinerea.
A serine/threonine kinase gene BcATG1 is involved in conidiation and sclerotial development in Botrytis cinerea.
复制标题
丝氨酸/苏氨酸激酶基因 BcATG1 参与灰葡萄孢 (Botrytis cinerea) 的分生孢子和菌核发育。
DOI：
10.1016/j.myc.2015.11.001
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发表时间：
2016
期刊：
影响因子：
1.4
通讯作者：
C. Tanaka
中科院分区：
文献类型：
--
作者：
Sumita T.;K. Izumitsu;C. Tanaka
灰葡萄孢是一种引起灰霉病的丝状子囊真菌。在最近的研究中，自噬已被证明是真菌生命周期的各个阶段所必需的。在这项研究中，我们的目的是调查自噬在生物学和农业重要阶段的B。灰霉病、分生孢子、菌核发育和植物侵染。我们鉴定并破坏了BcATG1基因，这是酵母自噬基因ATG1的直系同源物。BcATG 1的破坏引起对氮饥饿的敏感性。此外，干扰物在气生菌丝生长中表现出缺陷，与其他丝状真菌物种中的自噬缺陷突变体一致。这些结果表明，BcATG 1是真菌自噬功能所必需的。DBcATG1菌株也表明这两种类型的分生孢子减少。特别是，破坏剂表现出严重受损的大分生孢子的发展，并导致大分生孢子生产急剧减少。在低温条件下，菌核发育也受到影响的破坏。在致病性试验中，DBcATG1菌株在寄主植物上形成典型的病害症状。我们的研究结果表明，BcATG 1是两种类型的分生孢子和菌核发育所必需的，但不是致病性在B。© 2015 The Mycological Society of Japan.出版社：Elsevier BV版权所有。
Botrytis cinerea is a filamentous ascomycete fungus which is responsible for gray mold disease. In recent studies, autophagy has been shown to be required for various stages of the life cycle of fungi. In this study, we aimed to investigate the role of autophagy in biologically and agriculturally significant stages of B. cinerea, conidiation, sclerotial development and plant infection. We identified and disrupted the BcATG1 gene, an ortholog of the yeast autophagy gene ATG1. Disruption of BcATG1 caused sensitivity to nitrogen starvation. In addition, the disruptants showed defects in aerial-hyphal growth, consistent with autophagy-deficient mutants in other filamentous fungal species. These results suggested that BcATG1 is required for the function of autophagy in the fungus. DBcATG1 strains also indicated reduction in both two types of conidiation. Especially, the disruptants showed severely impaired macroconidiophore development and resulting drastic reduction in macroconidial production. Under cold conditions, sclerotial development was also affected in the disruptants. In pathogenicity tests, DBcATG1 strains formed typical disease symptoms on the host plant. Our results suggested that BcATG1 is required for two types of conidiation and sclerotial development, but not for pathogenicity in B. cinerea.© 2015 The Mycological Society of Japan. Published by Elsevier BV All rights reserved.