A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth
A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth
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真菌二萜合酶负责甾醇生物合成以促进生长
DOI：
10.3389/fmicb.2020.01426
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发表时间：
2020-07-10
影响因子：
5.2
通讯作者：
Zhu, Xudong
中科院分区：
文献类型：
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作者：
Liu, Yanjie;Duan, Anqing;Zhu, Xudong
小孢拟盘多毛孢菌（Pestalotiopsis microspora）中存在一个保守的开放阅读框架dps，它与功能研究较少的真菌二萜类糖苷酶具有显著的相似性。功能丧失法表明dps是该菌生长发育所必需的。缺失菌株，dps三角洲，表现出基本的生长迟缓，这可以故意恢复通过添加外源甾醇的媒体。气相色谱-质谱分析证实，失去了生产某些甾醇的能力。因此，dps Delta对几种胁迫条件的耐受性和抗性受损。次级代谢产物，如聚酮衍生物二苯并二氧杂环辛酮，显着减少。在分子水平上，dpseven的缺失影响了甲羟戊酸途径中基因的表达。这份报告增加了关于真菌二萜脱氢酶inPestalitiopsis microspora的知识。
A conserved open reading frame,dps, is described inPestalotiopsis microspora, sharing a remarkable similarity with fungal diterpene synthases whose function is less studied. Loss-of-function approach manifested thatdpswas necessary for the growth and the development of the fungus. A deletion strain,dps Delta, showed a fundamental retardation in growth, which could deliberately be restored by the addition of exogenous sterols to the media. Gas chromatography-mass spectrometry analysis confirmed the loss of the ability to produce certain sterols. Thus, the tolerance and the resistance ofdps Delta to several stress conditions were impaired. Secondary metabolites, such as the polyketide derivative dibenzodioxocinones, were significantly diminished. At the molecular level, the deletion ofdpseven affected the expression of genes in the mevalonate pathway. This report adds knowledge about fungal diterpene synthases inPestalitiopsis microspora.