In vitro and in vivo antagonism of pathogenic turf grass fungi by Streptomyces hygroscopicus strains YCED9 and WYE53
In vitro and in vivo antagonism of pathogenic turf grass fungi by Streptomyces hygroscopicus strains YCED9 and WYE53
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DOI：
10.1038/sj.jim.2900671
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发表时间：
1999-07-01
影响因子：
3.4
通讯作者：
Crawford, DL
中科院分区：
文献类型：
--
作者：
Chamberlain, K;Crawford, DL
疾病预防是目前用于尽量减少草坪和高尔夫球场草坪草真菌病害的做法。通过使用杀菌剂和定期清除茅草来实现预防。在利用木质纤维素降解性吸水链霉菌菌株 YCED9 和 WYE53 开发微生物生物脱茅产品的过程中，我们使用体外平板拮抗生物测定法证明，这两种菌株都是各种草坪草真菌病原体的拮抗剂。当培养物在茅草上生长时，这些活性就存在，如培养物滤液的抗真菌拮抗生物测定所证明的。使用生长室进行的实验表明，在沸石载体中含有 YCED9 和 WYE53 菌株孢子的生物去苔配方可以为肯塔基早熟禾幼苗提供针对草坪草病原体的保护，包括终极腐霉、尖孢镰刀菌、立枯丝核菌、核盘菌、禾谷全菌和 结果表明，通过将吸水链球菌 YCED9/WYE53 生物去苔配方的使用整合到常规草坪管理实践中，应该可以最大限度地减少茅草的堆积，同时还可以通过这些菌株的抗真菌生物防治活性来控制真菌性草坪病害。这反过来又有助于控制草坪草中的真菌病原体，同时最大限度地减少对草皮草的需求。 常规化学杀菌剂应用。
Disease prevention is a current practice used to minimize fungal diseases of turf grasses in lawns and golf greens. Prevention is accomplished through fungicide applications, and by periodic thatch removal. During the development of a microbial biodethatch product utilizing the lignocellulose-degrading Streptomyces hygroscopicus strains YCED9 and WYE53, we demonstrated using in vitro plate antagonism bioassays that both strains are antagonists of various turf grass fungal pathogens. These activities were present when the cultures were growing on thatch, as demonstrated by antifungal antagonism bioassays with culture filtrates. Experiments conducted using a growth chamber demonstrated that a bio-dethatch formulation containing spores of strains YCED9 and WYE53 in a zeolite carrier, provided protection for Kentucky bluegrass seedlings against turf grass pathogens, including Pythium ultimum, Fusarium oxysporum, Rhizoctonia solani, Sclerotinia homeocarpa, Gaeumannomyces graminis and Microdochium nivale, Results showed that by integrating the use of the S. hygroscopicus YCED9/WYE53 bio-dethatch formulation into routine turf management practices, it should be possible to both minimize thatch build-up while also controlling fungal turf grass diseases by way of the antifungal biocontrol activity of these strains, This in turn would help control fungal pathogens in turf grass while minimizing the need for routine chemical fungicide applications.