Branching patterns of rabbit oculomotor and trochlear nerves demonstrated by Sihler's stain technique
Branching patterns of rabbit oculomotor and trochlear nerves demonstrated by Sihler's stain technique
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DOI：
10.1080/714028170
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发表时间：
2002-01-01
影响因子：
1.6
通讯作者：
Elmas, C
中科院分区：
文献类型：
--
作者：
Gözil, R;Kadioglu, D;Elmas, C
采用改良的Sihler染色技术对动眼神经和滑车神经的分支模式进行可视化。从正常家兔眼中分离提睑肌、上直肌、下直肌、内直肌、下斜肌、上斜肌和滑车张肌，采用改良的Sihler技术进行处理。观察动眼神经和滑车神经的分布和末梢分支。详细显示了动眼神经的两个不同分支和末端分支，以及滑车神经的分布。Sihler的技术的应用使研究人员能够在相对透明的肌肉中追踪神经分支，而神经纤维是反染色的，清晰可见。该技术可用于眼外肌运动控制的详细研究。
A modified Sihler's stain technique was used to visualize the branching patterns of oculomotor and trochlear nerves. The levator palpebrae, superior rectus, inferior rectus, medial rectus, inferior oblique, superior oblique and tensor trochlea muscles were isolated from the eyes of normal rabbits and processed using modified Sihler's technique. The distributions and terminal ramifications of the oculomotor and trochlear nerves were observed. Two distinct divisions and terminal branches of the oculomotor nerve were shown in detail together with the trochlear nerve distribution. The application of Sihler's technique enables researchers to trace nerve branching within relatively transparent muscles, whereas the nerve fibers are counterstained and clearly visible. This technique could be useful for detailed studies of the motor control of extraocular muscles.