Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo.
Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo.
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发表时间：
1988-05
期刊：
影响因子：
4.6
通讯作者：
E. Hanneman;B. Trevarrow;W. K. Metcalfe;C. Kimmel;M. Westerfield
中科院分区：
文献类型：
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作者：
E. Hanneman;B. Trevarrow;W. K. Metcalfe;C. Kimmel;M. Westerfield
在斑马鱼胚胎的腹侧后脑和脊髓中，可以识别的第一批神经元以单细胞或小细胞簇的形式出现，它们周期性地分布，间隔等于体体的长度。在后脑中，存在一系列相应长度的神经粒，最早的神经元位于每个神经粒的中心。后脑和脊髓内的年轻神经元在活胚胎中进行了诺玛斯基光学鉴定，并通过组织化学方法标记乙酰胆碱酯酶活性和单克隆抗体zn-1识别的抗原表达。其中有单独识别的后脑网状脊髓神经元和脊髓运动神经元。这些观察结果表明，这些中枢神经系统区域的早期发育反映了一种共同的节段模式。随后，随着更多的神经元分化，这两个区域中最初相似的细胞模式发生分化。在脊髓中出现发育中的神经元的连续纵柱，而在后脑中则出现大小神经元簇的交替序列。
In the ventral hindbrain and spinal cord of zebrafish embryos, the first neurones that can be identified appear as single cells or small clusters of cells, distributed periodically at intervals equal to the length of a somite. In the hindbrain, a series of neuromeres of corresponding length is present, and the earliest neurones are located in the centres of each neuromere. Young neurones within both the hindbrain and spinal cord were identified in live embryos using Nomarski optics, and histochemically by labelling for acetylcholinesterase activity and expression of an antigen recognized by the monoclonal antibody zn-1. Among them are individually identified hindbrain reticulospinal neurones and spinal motoneurones. These observations suggest that early development in these regions of the CNS reflects a common segmental pattern. Subsequently, as more neurones differentiate, the initially similar patterning of the cells in these two regions diverges. A continuous longitudinal column of developing neurones appears in the spinal cord, whereas an alternating series of large and small clusters of neurones is present in the hindbrain.