Atomic force microscopy investigation of isolated virions of murine leukemia virus.
Atomic force microscopy investigation of isolated virions of murine leukemia virus.
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鼠白血病病毒分离病毒体的原子力显微镜研究。
DOI：
10.1128/jvi.79.3.1970-1974.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
McPherson,A
中科院分区：
文献类型：
--
作者：
Kuznetsov,YG;Low,A;Fan,H;McPherson,A
用原子力显微镜观察了在玻璃基质上传播的小鼠白血病病毒粒子。其粒径分布模式为145 nm，明显大于人类免疫缺陷病毒颗粒。颗粒大小的分布很广，这表明没有两个颗粒可能在含量或表面特征上完全相同。病毒粒子具有结节状突起，这可能代表从细胞膜出芽的痕迹。裂开的颗粒可以成像直径为65纳米的完整岩心。他们还允许估计病毒外壳厚度（35至40纳米），并显示在外壳和核心表面之间存在明显的凹槽。
Virions of mouse leukemia virus spread on glass substrates were visualized by atomic force microscopy. The size distribution mode was 145 nm, significantly larger than that for human immunodeficiency virus particles. The distribution of particle sizes is broad, indicating that no two particles are likely identical in content or surface features. Virions possess knoblike protrusions, which may represent vestiges of budding from cell membranes. Particles which split open allowed imaging of intact cores with diameters of 65 nm. They also permitted estimation of viral shell thickness (35 to 40 nm) and showed the presence of a distinct trough between the shell and the core surface.