EXCITATION AND DETECTION OF ACTION POTENTIAL-INDUCED FLUORESCENCE CHANGES THROUGH A SINGLE MONOMODE OPTICAL FIBER
EXCITATION AND DETECTION OF ACTION POTENTIAL-INDUCED FLUORESCENCE CHANGES THROUGH A SINGLE MONOMODE OPTICAL FIBER
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DOI：
10.1016/0167-4889(91)90214-i
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发表时间：
1991-01-10
期刊：
影响因子：
--
通讯作者：
KRAUTHAMER, V
中科院分区：
文献类型：
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作者：
BOWMASTER, TA;DAVIS, CC;KRAUTHAMER, V
已经开发出一种能够在体外检测单个细胞的细胞内电位变化的光学探针，该探针具有体内应用的潜力。 单模光纤将激光引导到用电压敏感荧光染料 WW781 染色的细胞上，并将部分产生的荧光返回到检测系统。 在这些初步实验中使用了体外青蛙心脏细胞。 从固定在零钙林格氏溶液中的心脏获得的跨膜电位移动 50 mV 时，荧光强度 10(-3) 的分数变化与其他光学方法报道的结果相当。 对于正常钙林格氏溶液中的心脏，检测到非常大的可重复运动相关伪影。
An optical probe capable of detecting intracellular potential changes in individual cells, in vitro, which has the potential for in vivo applications, has been developed. A single-mode optical fiber directs laser light onto cells stained with the voltage-sensitive fluorescent dye, WW781 and also returns part of the resulting fluorescence to a detection system. Frog cardiac cells in vitro were used in these initial experiments. The fractional change in fluorescent intensity of 10(-3) for a 50 mV shift in transmembrane potential obtained from a heart immobilized in zero calcium Ringer's solution is comparable to that reported for other optical methods. For hearts in normal calcium Ringer's solutions, very large reproducible motion related artifacts were detected.