UV RESONANCE RAMAN-SPECTROSCOPY FOR ANALYTICAL, PHYSICAL, AND BIOPHYSICAL CHEMISTRY .2.
UV RESONANCE RAMAN-SPECTROSCOPY FOR ANALYTICAL, PHYSICAL, AND BIOPHYSICAL CHEMISTRY .2.
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DOI：
10.1021/ac00052a001
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发表时间：
1993-02-15
影响因子：
7.4
通讯作者：
ASHER, SA
中科院分区：
文献类型：
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作者：
ASHER, SA
紫外共振拉曼光谱能够选择性地检测复杂混合物中特定物质的振动行为，这使得该技术在分析应用中具有独特的重要性。选择性由分析物组分与样品中存在的其他物质的相对拉曼截面确定。灵敏度取决于分析物拉曼谱带与样品的散射、干扰拉曼谱带和发射相比的相对强度。在低于260 nm的紫外光激发下，凝聚相中通常不会发生荧光干涉;那些最低单重态低于260 nm的物质，由于它们是柔性的，通过非辐射过程返回基态，所以荧光量子产率非常低（2）。
The ability of UV resonance Raman spectroscopy to selectively examine the vibrational behavior of particular species in complex mixtures makes the technique uniquely important for analytical applications. Selectivity is determined by the relative Raman cross sections of the analyte com-pared to other species present in the sample. Sensitivity depends on the relative intensities of the analyte Ra-man bands compared with overlap-ping, interfering Raman bands and emissions from the sample. Fluores-cence interference does not normally occur in condensed phases with UV excitation below 260 nm; those spe-cies that have their lowestsinglet state below 260 nm have vanishingly low fluorescence quantum yields be-cause they are flexible and return to the ground state through nonradia-tive processes (2).