Anti-Inflammatory Effect of Chloroform Fraction of Pyrus Ussuriensis Maxim. Leaf Extract on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis in nc/nga Mice
Anti-Inflammatory Effect of Chloroform Fraction of Pyrus Ussuriensis Maxim. Leaf Extract on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis in nc/nga Mice
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DOI：
10.3390/nu11020276
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发表时间：
2019-02-01
期刊：
影响因子：
5.9
通讯作者：
Kim, Sun Yeou
中科院分区：
文献类型：
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作者：
Cho, KyoHee;Kang, Min Cheol;Kim, Sun Yeou
在韩国被称为山梨的梨，在韩国、中国、日本被用作治疗哮喘、咳嗽、发烧的传统草药。乌苏里叶（PUL）对特应性皮炎（AD）有治疗作用。然而，没有关于PUL的特定成分的功效的报道。在本研究中，采用活性引导分离的方法来确定具有有效活性的化合物。采用高效液相色谱法（HPLC）鉴定了黄芪苷是其氯仿可溶性部分的主要成分。对黄芪黄芪苷和茯苓多糖进行体内体外抗AD作用试验。PULC和黄芪甲苷剂量依赖性地抑制小鼠巨噬细胞（RAW 264.7）细胞中一氧化氮（NO）的产生，以及肿瘤坏死因子(TNF-)/干扰素（IFN）诱导的HaCaT细胞中白细胞介素(IL)-6和IL-1的产生。在AD小鼠模型中，与2,4 -二硝基氯苯处理的NC/Nga小鼠相比，应用PULC和黄芪甲苷显著降低了皮炎严重程度、抓痕行为和表皮水分流失（TEWL）。此外，他们通过降低血清中的免疫球蛋白E （IgE）水平使皮肤屏障功能正常化。经PULC治疗后，HaCaT细胞和皮肤病变的聚丝蛋白和天青蛋白水平恢复正常。这些结果表明，在体外和体内动物模型中，PULC和黄芪甲苷通过减轻促炎细胞因子和免疫刺激来改善ad样症状。因此，PULC和黄芪甲苷可能是治疗AD的有效药物。
Pyrus ussuriensis Maxim, a pear commonly known as Sandolbae in Korea, is used as a traditional herbal medicine for asthma, cough, and fever in Korea, China, and Japan. P. ussuriensis Maxim leaves (PUL) have therapeutic effects on atopic dermatitis (AD). However, there are no reports on the efficacy of specific components of PUL. In the present study, activity-guided isolation of PUL was used to determine the compounds with potent activity. Astragalin was identified as the major component of the chloroform-soluble fraction of PUL (PULC) using High-performance liquid chromatography (HPLC) analysis. Astragalin and PULC were tested in vitro and in vivo for their effects against AD. PULC and astragalin dose-dependently inhibited the production of nitric oxide (NO) in mouse macrophage (RAW 264.7) cells, and interleukin (IL)-6 and IL-1 in tumor necrosis factor (TNF-)/interferon (IFN) induced HaCaT cells. In the AD mice model, PULC and astragalin application significantly reduced dermatitis severity, scratching behavior, and trans-epidermal water loss (TEWL) when compared to that of 2, 4-dinitrochlorobenzene-treated NC/Nga mice. Additionally, they normalized skin barrier function by decreasing immunoglobulin E (IgE) levels in the serum. Filaggrin and involucrin protein levels were normalized by PULC treatment in HaCaT cells and skin lesions. These results indicate that PULC and astragalin ameliorate AD-like symptoms by alleviating both pro-inflammatory cytokines and immune stimuli in vitro and in vivo in animal models. Therefore, PULC and astragalin might be effective therapeutic agents for the treatment of AD.