A proposed role for the SARS-CoV-2 nucleocapsid protein in the formation and regulation of biomolecular condensates
A proposed role for the SARS-CoV-2 nucleocapsid protein in the formation and regulation of biomolecular condensates
复制标题
DOI：
10.1096/fj.202001351
复制
发表时间：
2020-06-20
期刊：
影响因子：
4.8
通讯作者：
Ross, Eric D.
中科院分区：
文献类型：
--
作者：
Cascarina, Sean M.;Ross, Eric D.
到目前为止，最近发现的SARS-CoV-2病毒已经感染了全球超过690万人，并扰乱了全球经济。对SARS-CoV-2蛋白及其与宿主细胞蛋白相互作用的分子水平理解将有助于开发有效的SARS-CoV-2感染疫苗或治疗方法。SARS-CoV-2核衣壳（N）蛋白与SARS-CoV的N蛋白高度同源，N蛋白对于病毒RNA复制和包装成新的病毒体是必需的。新兴的模型表明，其他病毒的核衣壳蛋白可以形成生物分子凝聚物时空调节N蛋白的定位和功能。我们的生物信息学分析结合已有的实验证据表明，SARS-CoV-2 N蛋白能够通过与RNA和关键宿主细胞蛋白的相互作用在体内形成或调节生物分子缩合物。我们讨论了多种模型，其中SARS冠状病毒-2的N蛋白可能利用这种活动来调节病毒的生命周期和宿主细胞对病毒感染的反应。
To date, the recently discovered SARS-CoV-2 virus has afflicted >6.9 million people worldwide and disrupted the global economy. Development of effective vaccines or treatments for SARS-CoV-2 infection will be aided by a molecular-level understanding of SARS-CoV-2 proteins and their interactions with host cell proteins. The SARS-CoV-2 nucleocapsid (N) protein is highly homologous to the N protein of SARS-CoV, which is essential for viral RNA replication and packaging into new virions. Emerging models indicate that nucleocapsid proteins of other viruses can form biomolecular condensates to spatiotemporally regulate N protein localization and function. Our bioinformatic analyses, in combination with pre-existing experimental evidence, suggest that the SARS-CoV-2 N protein is capable of forming or regulating biomolecular condensates in vivo by interaction with RNA and key host cell proteins. We discuss multiple models, whereby the N protein of SARS-CoV-2 may harness this activity to regulate viral life cycle and host cell response to viral infection.