RECOMBINANT INTERLEUKIN 2-ACTIVATED NATURAL-KILLER-CELLS REGULATE IGG2A PRODUCTION
RECOMBINANT INTERLEUKIN 2-ACTIVATED NATURAL-KILLER-CELLS REGULATE IGG2A PRODUCTION
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DOI：
10.1002/eji.1830200824
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发表时间：
1990-08-01
影响因子：
5.4
通讯作者：
TEILLAUD, JL
中科院分区：
文献类型：
--
作者：
AMIGORENA, S;BONNEROT, C;TEILLAUD, JL
本文分析了重组白介素2(RIL2)和rIL2激活的自然杀伤(NK)细胞对内毒素(LPS)刺激的裸小鼠脾细胞产生免疫球蛋白亚型的影响。我们发现，rIL-2诱导的IgG2a分泌呈剂量依赖性增加，同时对其他Ig亚型的分泌有抑制作用。对脂多糖和脂多糖+rIL-2刺激的裸小鼠脾细胞表型的分析表明，在rIL-2存在的情况下，出现了Thy-1+asialo GM-1+slgM-CD3-CD4-CD8-细胞群，而单独用rIL-2刺激裸鼠脾细胞产生了类似表型的细胞群。这些细胞裂解YAC-1细胞，不含α。或者伽马。在共培养实验中，这些细胞选择性地增加了脂多糖刺激的裸小鼠脾细胞分泌IgG2a的能力。由rIL-2激活的NK细胞和rIL-2激活的NK细胞所触发的IgG2a诱导，以及由rIL-2所触发的诱导，均被抗干扰素γ阻断。单抗。因此，rIL-2激活的NK细胞通过分泌干扰素-γ来增强IgG2a的产生。
In this report we have analyzed the effect of recombinant interleukin 2 (rIL2) and rIL 2-activated natural killer (NK) cells on the production of immunoglobulin isotypes by lipopolysaccharide (LPS)-stimulated spleen cells from nude mice. We found that rIL 2 induced a dose-dependent increase of IgG2a secretion and a concomitant inhibition of the secretion of other Ig isotypes. The analysis of the phenotype of LPS- and LPS+rIL 2-stimulated nude spleen cells showed the appearance of a Thy-1+asialo GM-1+slgM-CD3-CD4-CD8- cell population in the presence of rIL 2. A population with a similar phenotype was generated upon stimulation of spleen cells from nude mice with rIL 2 alone. These cells lysed YAC-1 cells, did not contain the .alpha. or .gamma. transcripts encoding the corresponding T cell receptor chains and are therefore NK cells activated by rIL 2. In co-culture experiments, these cells selectively increased the secretion of IgG2a by LPS-stimulated splenocytes from nude mice. The IgG2a induction triggered by rIL 2-activated NK cells, as well as that triggered by riL 2, were blocked by an anti-interferon .gamma. monoclonal antibody. Thus, rIL 2 activated-NK cells enhance the production of IgG2a by secreting interferon-.gamma.