Facial paralysis induced by ear inoculation of herpes simplex virus in rat
Facial paralysis induced by ear inoculation of herpes simplex virus in rat
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DOI：
10.1016/j.anl.2016.04.002
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发表时间：
2017-02-01
影响因子：
1.7
通讯作者：
Hato, Naohito
中科院分区：
文献类型：
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作者：
Fujiwara, Takashi;Matsuda, Seiji;Hato, Naohito
目的：Bell麻痹是由单纯疱疹病毒1型(HSV-1)重新激活引起的。使用接种了HSV-1 Kos毒株的Balb/c小鼠，我们之前开发了一种模拟轻度贝尔麻痹的动物疾病模型。本研究建立了一种比小鼠模型更严重的面神经麻痹动物模型。方法：3周龄雌性Wister大鼠，体重60-80g，耳廓接种HSV-1，腹腔注射阿昔洛韦灭活感染的HSV-1。用磷酸盐缓冲液代替HSV-1接种作为阴性对照。采用定量聚合酶链式反应(PCR)、行为测试(瞬目反射)、神经电生理、周围神经组织病理学、面神经核免疫组织化学等方法进行检测。结果：面瘫在接种病毒后3~5d出现，病情发展至7d。定量聚合酶链式反应显示面神经内HSV-1DNA拷贝数在24~72h呈上升趋势，提示HSV-1感染发生在神经内。单纯疱疹病毒1型感染组和对照组大鼠的神经电图值分别为33.0+/-15.3%和110.0+/-18.0%。周围神经组织病理学表现为面神经脱髓鞘、脱失，面神经核变性。结论：耳廓接种HSV-1 Kos毒株后，Wistar大鼠面神经麻痹。行为学、组织病理学和神经电描记术数据表明，在先前的小鼠疾病模型中，我们的大鼠面神经麻痹的严重程度比动物更严重。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Objective: Bell's palsy is caused by the reactivation of herpes simplex virus type 1 (HSV-1). Using Balb/c mice inoculated with the KOS strain of HSV-1, we previously developed an animal disease model that simulated mild Bell's palsy. The current study developed an animal disease model of more severe facial palsy than that seen in the mouse model.Methods: Three-week-old female Wister rats weighing 60-80 g were inoculated on the auricle with HSV-1 and acyclovir was administered intraperitoneally to deactivate the infected HSV-1. Instead of HSV-1, phosphate-buffered saline was used for inoculation as a negative control. Quantitative polymerase chain reaction (PCR), behavior testing (blink reflex), electroneuronography, histopathology of the peripheral nerve, and immunohistochemistry of the facial nerve nucleus were evaluated.Results: Facial palsy occurred 3-5 days after virus inoculation, and the severity of the facial palsy progressed for up to 7 days. Quantitative PCR showed an increase in HSV-1 DNA copies in the facial nerve from 24 to 72 h, suggesting that HSV-1 infection occurred in the nerve. Electroneuronography values were 33.0 +/- 15.3% and 110.0 +/- 18.0% in HSV-1-inoculated and control rats, respectively. The histopathology of the peripheral nerve showed demyelination and loss of the facial nerve, and the facial nerve nucleus showed degeneration.Conclusion: Facial palsy developed in Wister rats following inoculation of the KOS strain of HSV-1 onto the auricles. The behavioral, histopathological, and electroneuronography data suggested that the severity of facial palsy was greater in our rats than in animals in the previous mouse disease model. (C) 2016 Elsevier Ireland Ltd. All rights reserved.