Microscopy with Microlens Arrays: High Throughput, High Resolution and Light-field Imaging References and Links
Microscopy with Microlens Arrays: High Throughput, High Resolution and Light-field Imaging References and Links
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通讯作者：
A. Orth;K. Crozier;J. R. Pepperkok;Ellenberg;E Schonbrun;A. R. Abate;P. Steinvurzel;D. A. Weitz-D.
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作者：
A. Orth;K. Crozier;J. R. Pepperkok;Ellenberg;E Schonbrun;A. R. Abate;P. Steinvurzel;D. A. Weitz-D.
我们展示了高度平行化的荧光扫描显微镜使用折射微透镜阵列。荧光珠和大鼠股骨组织在5.5 mm x 5.5 mm的视场内成像，像素吞吐量高达4百万像素/秒，分辨率为706 nm。我们还展示了提取一堆微球的不同透视图的能力。使用集成波带板阵列的高通量荧光检测，“使用衍射微透镜阵列的微流体荧光测量系统，“用于数字显微镜的简单而强大的基于图像的自动聚焦，“用于超快速虚拟载玻片处理和远程病理学的阵列显微镜。设计、制造和验证研究，“基于全息聚焦网格照明的宽视场显微镜，“具有塔尔博特图案照明的宽视场显微镜中的光学焦平面调谐“，“光学用于成像微米尺寸物体的双重微透镜阵列“，J. Micromech.使用短焦距菲涅耳波带片的扫描显微镜，“Opt. Theoretical analysis of confocal microscopy with microlenses“，Appl. Design，fabrication and testing of microlens arrays for sensors and microsystems，“Pure Appl. Surface profiles of reflow microlenses under the influence of surface tension and gravity“，Opt. Accurate detection and complete tracking of large population of features in three dimensions，“原子力显微镜闭环压电平移台的光学控制“，Rev.微制造水浸波带片光镊，“应用。
We demonstrate highly parallelized fluorescence scanning microscopy using a refractive microlens array. Fluorescent beads and rat femur tissue are imaged over a 5.5 mm x 5.5 mm field of view at a pixel throughput of up to 4 megapixels/s and a resolution of 706 nm. We also demonstrate the ability to extract different perspective views of a pile of microspheres. High-throughput fluorescence detection using an integrated zone-plate array, " A microfluidic fluorescence measurement system using an astigmatic diffractive microlens array, " Opt. Simple and robust image-based autofocusing for digital microscopy, " Opt. An array microscope for ultrarapid virtual slide processing and telepathology. Design, fabrication, and validation study, " Hum. Wide field-of-view microscope based on holographic focus grid illumination, " Opt. Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination, " Opt. A doublet microlens array for imaging micron-sized objects, " J. Micromech. Scanning microscopy using a short-focal-length fresnel zone plate, " Opt. Theoretical analysis of confocal microscopy with microlenses, " Appl. Design, fabrication and testing of microlens arrays for sensors and microsystems, " Pure Appl. Surface profiles of reflow microlenses under the influence of surface tension and gravity, " Opt. Accurate detection and complete tracking of large populations of features in three dimensions, " Opt. control of a closed-loop piezoelectric translation stage for atomic force microscope, " Rev. Microfabricated water immersion zone plate optical tweezer, " Appl.