Exchange of RNA polymerase II initiation and elongation factors during gene expression in vivo
Exchange of RNA polymerase II initiation and elongation factors during gene expression in vivo
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DOI：
10.1016/s1097-2765(02)00502-6
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发表时间：
2002-04-01
期刊：
影响因子：
16
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Pokholok, DK;Hannett, NM;Young, RA
我们系统地研究了酵母中RNA聚合酶II转录起始和延伸装置中的启动子和开放阅读框在体内的占有率。RNA聚合酶II、介体和通用转录因子(GTF)被招募到所有基因激活测试的启动子中。RNA聚合酶II、TFIIS、Spt5，以及出人意料的Paf1/CDC73复合体，被发现与开放阅读框架相关。体内ORF上Paf1/CDC73复合体的存在表明该复合体在伸长过程中具有新的功能。在所测试的任何条件下均未检测到伸长子，进一步分析表明，大部分伸长子是细胞质的。这些结果为活细胞中的转录启动和延长装置提出了一个修正的模型。
We have systematically explored the in vivo occupancy of promoters and open reading frames by components of the RNA polymerase II transcription initiation and elongation apparatuses in yeast. RNA polymerase II, Mediator, and the general transcription factors (GTFs) were recruited to all promoters tested upon gene activation. RNA polymerase II, TFIIS, Spt5, and, unexpectedly, the Paf1/Cdc73 complex, were found associated with open reading frames. The presence of the Paf1/Cdc73 complex on ORFs in vivo suggests a novel function for this complex in elongation. Elongator was not detected under any conditions tested, and further analysis revealed that the majority of elongator is cytoplasmic. These results suggest a revised model for transcription initiation and elongation apparatuses in living cells.