Different interaction between HIV-1 Vif and its cellular target proteins APOBEC3G/APOBEC3F
Different interaction between HIV-1 Vif and its cellular target proteins APOBEC3G/APOBEC3F
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DOI：
10.2152/jmi.57.89
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发表时间：
2010-02-01
影响因子：
0.7
通讯作者：
Adachi, Akio
中科院分区：
文献类型：
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作者：
Nagao, Tamiko;Yamashita, Tomoki;Adachi, Akio
我们研究了一系列人类免疫缺陷病毒1型（HIV-1）Vif定点突变体与细胞抗病毒因子APOBEC 3G/APOBEC 3F的相互作用。通过单循环感染性试验监测，在H9细胞中显示生长缺陷的突变病毒无一例外地不能有效抵消APOBEC 3G和/或APOBEC 3F。虽然Vif C端区域的生长缺陷突变体不能抑制APOBEC 3G/APOBEC 3F，但一些N端区域突变体确实中和了APOBEC 3G/APOBEC 3F之一。这些数据表明，APOBEC 3家族中除APOBEC 3G/APOBEC 3F以外的成员对于抗HIV-1活性并不重要。此外，通过平衡密度离心分析，发现APOPEC 3G/APOBEC 3F与病毒粒子中的Vif不同地缔合。综上所述，这些结果表明HIV-1 Vif和APOBEC 3G之间的相互作用不同于Vif和APOBEC 3F之间的相互作用。
We examined a series of site-directed point mutants of human immunodeficiency virus type 1 (HIV-1) Vif for their interaction with cellular anti-viral factors APOBEC3G/APOBEC3F. Mutant viruses that display growth-defect in H9 cells did not counteract effectively APOBEC3G and/or APOBEC3F without exception, as monitored by single-cycle infectivity assays. While growth-defective mutants of Vif C-terminal region were unable to suppress APOBEC3G/APOBEC3F, some N-terminal region mutants did neutralize one of APOBEC3G/APOBEC3F. These data have suggested that members of APOBEC3 family other than APOBEC3G/APOBEC3F are not important for anti-HIV-1 activity. Furthermore, APOPEC3G/APOBEC3F were found to differently associate with Vif in virions as analyzed by equilibrium density centrifugation. Taken together, these results indicated that interaction of HIV-1 Vif and APOBEC3G is distinct from that between Vif and APOBEC3F.