Prognostic score of gastric cancer determined by cDNA microarray.
Prognostic score of gastric cancer determined by cDNA microarray.
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发表时间：
2002-11
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通讯作者：
H. Inoue;A. Matsuyama;K. Mimori;H. Ueo;M. Mori
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作者：
H. Inoue;A. Matsuyama;K. Mimori;H. Ueo;M. Mori
目的为了更全面地评估胃癌患者的预后，我们尝试开发一种利用cDNA微阵列的预后评分系统。实验设计从43例胃癌患者的肿瘤/正常配对样本中提取RNA，并进行cDNA微阵列杂交。结果我们选择了 78 个基因，这些基因在攻击性组和非攻击性组之间在 5 个常规病理因素方面存在差异表达。接下来，我们确定每个基因的系数。此后，通过总结每个基因的值来计算预后评分。其范围为 -47 至 201，中位数为 114。其分布有两个峰值。术后 5 年以上仍存活且无复发证据的 11 名患者中有 10 名得分为 100 分。 13名存活但随访时间为100分的患者在随访期间发现疾病复发。结论这些研究结果表明，这种带有cDNA微阵列的系统有助于全面分析肿瘤的恶性行为，并可能提供准确的预后信息。
PURPOSE To estimate gastric cancer patients' prognosis more comprehensively,we tried to develop a prognostic scoring system using a cDNA microarray. EXPERIMENTAL DESIGN RNA was extracted from tumor/normal paired samples of 43 patients with gastric cancer, and cDNA microarray hybridization was performed. RESULTS We selected 78 genes that were differentially expressed between aggressive and nonaggressive groups with respect to five conventional pathological factors. Next, we determined a coefficient for each gene. Thereafter a prognostic score was calculated by summing-up the value for each gene. It ranged from -47 to 201 with a median of 114. There were two peaks in its distribution. Ten of 11 patients who were alive with no evidence of recurrence >5 years after the operation showed a score of 100 points. In 13 patients who were alive but the follow-up time was 100 points revealed recurrent disease during the follow-up. CONCLUSIONS These findings demonstrate that such a system with cDNA microarray can contribute to the comprehensive analysis of malignant behavior of the tumor and may provide accurate information on prognosis.