A new family of plant transcription factors displays a novel ssDNA-binding surface
A new family of plant transcription factors displays a novel ssDNA-binding surface
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DOI：
10.1038/nsb814
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发表时间：
2002-07-01
期刊：
影响因子：
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通讯作者：
Sygusch, J
中科院分区：
文献类型：
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作者：
Desveaux, D;Allard, J;Sygusch, J
p24 是植物防御转录因子 PBF-2 的单链 DNA (ssDNA) 结合亚基，其晶体结构已确定为 2.3 埃分辨率。 p24 是普遍存在的植物特异性蛋白的一个新家族的代表，由于其四级结构，我们将其称为 Whirly 家族。 PBF-2 由四个 p24 分子组成，它们通过螺旋-环-螺旋基序相互作用。这种相互作用产生了一个中心孔，β-链向外辐射，导致四级结构呈现出旋转的外观。 p24 亚基的非晶体学 C-4 对称排列对于 ssDNA 结合蛋白来说是新颖的，并且可以解释 PBF-2 的结合特异性。这种结构排列也支持 PBF-2 在结合熔化的启动子区域以调节基因表达中的作用。
The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 Angstrom resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C-4 symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression.