The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: Role of vagal innervation
The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: Role of vagal innervation
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DOI：
10.1016/j.surg.2005.09.002
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发表时间：
2006-04-01
期刊：
影响因子：
3.8
通讯作者：
Sarr, MG
中科院分区：
文献类型：
--
作者：
Houghton, SG;Zarroug, AE;Sarr, MG
背景在大鼠空肠中，己糖转运蛋白钠依赖性葡萄糖转运蛋白1、葡萄糖转运蛋白2和葡萄糖转运蛋白5的蛋白质和信使RNA（mRNA）水平遵循（每日）昼夜节律。由于迷走神经支配介导的其他蛋白质在大鼠小肠的昼夜活动，我们假设这些己糖转运蛋白的mRNA和蛋白质水平的昼夜变化是由迷走神经支配介导的。将48只大鼠置于严格维持的、交替12小时的光暗室中，进行假剖腹术（n = 24）或双侧全腹部迷走神经切断术（n = 24）。术后4周，每组6只大鼠在3（AM），9（AM），3（PM）和9（PM）收获空肠粘膜;通过逆转录实时聚合酶链反应和蛋白质水平半定量Western印迹分析测定mRNA水平。转运蛋白mRNA和蛋白质水平表示为与稳定表达的管家基因甘油醛-6-磷酸脱氢酶的相应mRNA和蛋白质水平的比率。结果。在假手术对照组中，所有3种己糖转运蛋白的mRNA和蛋白水平均显示出昼夜变化（P <0.10）。迷走神经支配出现差异介导的昼夜变化，在大鼠空肠己糖转运蛋白的mRNA和蛋白质表达的转录后，和/或翻译后过程。
Background. Protein and messenger RNA (mRNA) levels of the hexose transporters sodium-dependent glucose transporter-1, glucose transporter 2 and glucose transporter 5 follow a (daily) diurnal rhythm in rat jejunum. Because vagal innervation mediates the diurnal activity of other proteins in the rat small bowel, we hypothesized that the diurnal variation of mRNA and protein levels of these hexose transport proteins are mediated by vagal innervation.Methods. Forty-eight rats kept in a strictly maintained, alternating 12-hour light-dark room underwent either sham laparotomy (n = 24) or bilateral total abdominal vagotomy (n = 24). Four weeks post-operatively, jejunal mucosa was harvested from 6 rats in each group at 3 (AM), 9 (AM), 3 (PM) and 9 (PM); mRNA levels were determined by reverse transcription real-time polymerase chain reaction and protein levels by semiquantitative Western blot analysis. Transporter mRNA and protein levels were expressed as a ratio to the corresponding mRNA and protein levels of the stably expressed housekeeping gene glyceraldehyde-6-phosphate dehydrogenase.Results. mRNA and protein levels for all 3 hexose transporters showed diurnal variation in sham controls (P .10 for all).Conclusions. Vagal innervation appears to differentially mediate the diurnal changes in hexose transporter mRNA and protein expression in the rat jejunum by posttranscriptional, and/or posttranslational processes.