Human antibodies against formaldehyde-human serum albumin conjugates or human serum albumin in individuals exposed to formaldehyde.
Human antibodies against formaldehyde-human serum albumin conjugates or human serum albumin in individuals exposed to formaldehyde.
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暴露于甲醛的个体中抗甲醛-人血清白蛋白缀合物或人血清白蛋白的人抗体。
DOI：
10.1159/000233942
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Harris,KE
中科院分区：
文献类型：
--
作者：
Patterson,R;Pateras,V;Grammer,LC;Harris,KE
对使用甲醛（F）灭菌透析器进行血液透析的患者血清进行研究，以确定是否可以检测到与人血清白蛋白（HSA）结合的抗F抗体。使用不沉淀HSA的F与HSA的比率制备F-人血清白蛋白（F-HSA）缀合物。与HSA相比，F-HSA缀合物的迁移与HSA不同，其中F-HSA的负电荷增加。F-HSA用于ELISA。结果表明，在某些血清中可检测到抗F-HSA的IgG、IgM、伊加和IgE抗体。在最高滴度的血清中，显示IgG抗体不是针对单独的F或F-赖氨酸，而是针对F-HSA的抗原分组。在这一系列透析患者中，未发现IgG或IgE抗体与免疫复合物或过敏反应相关。部分透析患者血清具有抗HSA抗体活性。2名鼻炎医生暴露于氟后的血清对F-HSA或HSA没有抗体活性。两名有氟诱导哮喘病史的护士没有IgG抗体，但有抗F-HSA和HSA的IgE抗体。该免疫应答谱类似于用F或F犬白蛋白免疫的犬中的应答。我们还没有能够确定其他半抗原-HSA化合物反应的患者中的抗HSA抗体，这表明抗HSA抗体在F暴露的人可能与F暴露。
Sera from patients undergoing hemodialysis with formaldehyde (F)-sterilized dialyzers were studied to determine if antibodies against F conjugated to human serum albumin (HSA) could be detected. F-human serum albumin (F-HSA) conjugates were prepared using ratios of F to HSA that did not precipitate the HSA. The F-HSA conjugates migrate differently electrophoretically than HSA with an increased negative charge of F-HSA as compared with HSA. The F-HSA was used in an ELISA. The results demonstrated that in certain sera, IgG, IgM, IgA and IgE antibodies against F-HSA could be measured. In the highest titered sera, it was shown that the IgG antibody was not directed against F alone or F-lysine but against an antigenic grouping of F-HSA. No correlation of either IgG or IgE antibodies with immune complex or allergic reactions was found in this series of dialysis patients. Some sera from dialysis patients had antibody activity against HSA. Sera from 2 physicians with rhinitis after F exposure had no antibody activity against F-HSA or HSA. Two nurses with a history of F-induced asthma had no IgG antibodies but did have IgE antibodies against F-HSA and HSA. This spectrum of immunologic responses is analogous to responses in dogs immunized with F or F dog albumin. We have not been able to identify anti HSA antibodies in patients reactive to other hapten-HSA compounds and it is suggested that anti HSA antibodies in F-exposed humans may relate to the F exposure.