Thermosensitive display of carbohydrate ligands on microgels for switchable binding of proteins and bacteria.
Thermosensitive display of carbohydrate ligands on microgels for switchable binding of proteins and bacteria.
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DOI：
10.1021/acsami.9b08537
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发表时间：
2019-07
影响因子：
9.5
通讯作者：
T. Paul;Sophie Rübel;Marco Hildebrandt;A. K. Strzelczyk;Carina Spormann;T. Lindhorst;Stephan Schmidt
中科院分区：
文献类型：
--
作者：
T. Paul;Sophie Rübel;Marco Hildebrandt;A. K. Strzelczyk;Carina Spormann;T. Lindhorst;Stephan Schmidt
报道了糖功能化的温敏性聚（N-异丙基丙烯酰胺）微凝胶的合成及其在温度切换时结合糖结合病原体的能力。发现微凝胶的结合亲和力在其较低临界溶液温度（LCST）以上增加，使得能够热致变色地捕获病原体。在此，通过单个聚合步骤实现了一系列具有低于1摩尔%的相对低的甘露糖官能化程度的微凝胶。随着甘露糖密度的增加，微凝胶尺寸增加，LCST降低至26°C。与伴刀豆球蛋白A的聚类表明结合亲和力通过较高的甘露糖含量和通过将温度升高到LCST以上而增强。与E.大肠杆菌证实了更强的特异性相互作用高于LCST和形成机械稳定的聚集体，使有效的分离E.大肠杆菌中分离纯化。对于高于LCST的小温育时间，也证实了微凝胶在低于LCST时再次释放病原体的潜力。与现有的可切换支架相比，几乎完全由热敏材料组成的微凝胶经历了很大的体积变化，这使得它们能够极大地改变配体的密度，从而在捕获和释放之间切换。这是简单的，但新的方法可能与广泛的生物活性配体兼容。因此，热敏微凝胶代表了一个有前途的平台，用于特异性捕获或释放细胞或病原体。
The synthesis of carbohydrate-functionalized thermosensitive poly(N-isoproylacrylamide) microgels and their ability to bind carbohydrate-binding pathogens upon temperature switch is reported. It is found that the microgels' binding affinity is increased above their lower critical solution temperature (LCST) enabling thermo-triggerable capture of pathogens. Here a series of microgels with comparatively low mannose functionalization degrees below 1 mol % is achieved by a single polymerization step. Upon increase of mannose density, the microgel size increases and the LCST decreases to 26°C. Clustering with concanavalin A indicated that binding affinity is enhanced by a higher mannose content and by raising the temperature above the LCST. Binding studies with E. coli confirm stronger specific interactions above the LCST and formation of mechanically stable aggregates enabling efficient separation of E. coli by filtration. For small incubation times above LCST, the microgels' potential to release pathogens again below LCST is confirmed as well. Compared to existing switchable scaffolds, microgels nearly entirely composed of thermosensitive material undergo a large change in volume, which allows them to drastically vary the density of ligands to switch between capture and release. This is straightforward, yet novel approach is likely compatible with a broad range of bioactive ligands. Therefore, thermosensitive microgels represent a promising platform for the specific capture or release of cells or pathogens.