Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids
Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids
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DOI：
10.1266/ggs.86.295
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发表时间：
2011-10-01
影响因子：
1.1
通讯作者：
Matsumoto, Kouji
中科院分区：
文献类型：
--
作者：
Matsuoka, Satoshi;Chiba, Minako;Matsumoto, Kouji
将具有破坏的ugtP的枯草芽孢杆菌马尔堡168细胞弯曲和膨胀，该ugtP编码参与糖脂合成的UDP-葡糖基转移酶。在ugtP突变体细胞中，发现细胞质外功能σ SigM、SigV和SigX被激活。将sigM的破坏等位基因引入ugtP菌株中导致甚至更异常的形态，细胞呈气球样形状;这些细胞在LB培养基中的生长通过加入1.5%NaCl而受到阻碍。MgSO 4或MnCl 2的加入抑制了异常形貌。在ugtP突变细胞中，来自maf（指定为Pupstream mreB）和PmreBH中的上游启动子的mreB操纵子的转录分别高4.3倍和2.3倍，并且GFP-MreB的定位不是离散的点（在明显的螺旋模式中），而是微弱的和不规则的簇。GFP-MreB蛋白在ugtP突变体细胞中减少。我们认为，糖脂是重要的MreB亚型采取的配置，出现离散点，并发挥作用，在塑造成直杆细胞。
Bacillus subtilis Marburg 168 cells with disrupted ugtP, which encodes UDP-glucosyltransferase involved in glucolipid synthesis, were bent and distended. In the ugtP mutant cells, the extracytoplasmic function sigmas SigM, SigV and SigX, were found to be activated. Introduction of a disrupted allele of sigM into the ugtP strain caused even more abnormal morphology, with cells taking on a balloon-like shape; growth of these cells in LB medium was hampered by addition of 1.5% NaCl. Addition of MgSO4 or MnCl2 suppressed the abnormal morphology. In ugtP mutant cells the transcription of the mreB operon from an upstream promoter in maf (designated Pupstream mreB) and PmreBH was 4.3- and 2.3-fold higher, respectively, and localization of GFP-MreB was not in discrete dots (in an apparently helical pattern), but faint and in irregular clusters. GFP-MreB protein was reduced in the ugtP mutant cells. We suggest that glucolipids are important for MreB isoforms to take on the configuration that appears as discrete dots and plays a role in shaping cells into straight rods.