Identification of microRNAs regulated by tobacco curly shoot virus co-infection with its betasatellite in Nicotiana benthamiana
Identification of microRNAs regulated by tobacco curly shoot virus co-infection with its betasatellite in Nicotiana benthamiana
复制标题
烟草卷枝病毒与其β卫星共感染调控的microRNA的鉴定
DOI：
10.1186/s12985-019-1234-5
复制
发表时间：
2019-11-07
期刊：
影响因子：
4.8
通讯作者：
Qing, Ling
中科院分区：
文献类型：
--
作者：
Du, Jiang;Wu, Gentu;Qing, Ling
背景microRNAs(MiRNAs)是一类21-24个核苷酸的内源非编码小RNA，在植物发育和对生物和非生物胁迫的防御反应中发挥重要作用。烟草曲茎病毒(TbCSV)是茄科植物上引起卷叶和植株矮化症状的一种单份卷叶病毒。TbCSV的β卫星(TbCSB)与TbCSV共侵染时，症状加重，病毒积累加快。方法利用高通量测序技术对TbCSV和TbCSB共感染本氏烟草所调控的miRNAs进行序列分析。结果小RNA序列分析表明，在TbCSV和TbCSB共侵染的本底夜蛾植株中，共有13个已知的miRNAs和42个新的miRNAs差异表达。根据基因本体论(GO)分析，通过数据挖掘发现了几个潜在的miRNA靶向基因，这些基因除了参与植物对病毒感染的防御机制外，还参与了催化和代谢过程。此外，通过实时定量聚合酶链式反应(qRT-PCR)方法验证了几个差异表达的miRNAs及其miRNA靶向基因的表达。结论已鉴定出大量miRNAs，并对其靶基因、功能注释进行了探索。我们的研究结果为进一步了解TbCSV和TbCSB共感染后的miRNA调控机制提供了信息。
Background MicroRNAs (miRNAs) are a class of 21-24 nucleotide endogenous non-coding small RNAs that play important roles in plant development and defense responses to biotic and abiotic stresses. Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus, cause leaf curling and plant stunting symptoms in many Solanaceae plants. The betasatellite of TbCSV (TbCSB) induces more severe symptoms and enhances virus accumulation when co-infect the plants with TbCSV. Methods In this study, miRNAs regulated by TbCSV and TbCSB co-infection in Nicotiana benthamiana were characterized using high-throughput sequencing technology. Results Small RNA sequencing analysis revealed that a total of 13 known miRNAs and 42 novel miRNAs were differentially expressed in TbCSV and TbCSB co-infected N. benthamiana plants. Several potential miRNA-targeted genes were identified through data mining and were involved in both catalytic and metabolic processes, in addition to plant defense mechanisms against virus infections according to Gene Ontology (GO) analyses. In addition, the expressions of several differentially expressed miRNAs and their miRNA-targeted gene were validated through quantitative real time polymerase chain reaction (qRT-PCR) approach. Conclusions A large number of miRNAs are identified, and their target genes, functional annotations also have been explored. Our results provide the information on N. benthamiana miRNAs and would be useful to further understand miRNA regulatory mechanisms after TbCSV and TbCSB co-infection.