FGF-dependent generation of oligodendrocytes by a hedgehog-independent pathway
FGF-dependent generation of oligodendrocytes by a hedgehog-independent pathway
复制标题
DOI：
10.1242/dev.00871
复制
发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Allen, ND
中科院分区：
文献类型：
--
作者：
Chandran, S;Kato, H;Allen, ND
在发育过程中，脊髓少突胶质细胞前体（OPC）起源于腹侧，而不是背侧，神经上皮。Sonic hedgehog（SHH）对脊髓腹侧区的少突胶质细胞产生具有至关重要的作用;然而，关于SHH信号传导和神经干细胞（NSC）的少突胶质细胞生成知之甚少。我们发现，从脊髓背侧分离的神经干细胞可以产生少突胶质细胞FGF 2治疗后，MAP激酶依赖的现象，是与诱导的专性寡基因Olig 2。环巴胺，一种有效的刺猬信号抑制剂，没有阻止从FGF 2处理的神经球培养物中形成少突胶质细胞。此外，从SHH无效小鼠产生的神经球也产生少突胶质细胞，即使在环巴胺的存在下。这些发现与神经干细胞生成少突胶质细胞的刺猬独立途径的想法是一致的。
During development, spinal cord oligodendrocyte precursors (OPCs) originate from the ventral, but not dorsal, neuroepithelium. Sonic hedgehog (SHH) has crucial effects on oligodendrocyte production in the ventral region of the spinal cord; however, less is known regarding SHH signalling and oligodendrocyte generation from neural stem cells (NSCs). We show that NSCs isolated from the dorsal spinal cord can generate oligodendrocytes following FGF2 treatment, a MAP kinase dependent phenomenon that is associated with induction of the obligate oligogenic gene Olig2. Cyclopamine, a potent inhibitor of hedgehog signalling, did not block the formation of oligodendrocytes from FGF2-treated neurosphere cultures. Furthermore, neurospheres generated from SHH null mice also produced oligodendrocytes, even in the presence of cyclopamine. These findings are compatible with the idea of a hedgehog independent pathway for oligodendrocyte generation from neural stem cells.